Study of the interactions between S. pasteurii and indigenous bacteria and the effect of these interactions on the MICP

  • Peng LiuEmail author
  • Guang-hui Shao
  • Rong-pin Huang
Original Paper


A new soil improvement method, called microbial-induced calcite precipitation (MICP), has recently emerged, which is based on microbial mineralisation by injecting bacteria solution and nutrient into soil for the purpose of modifying physico-mechanical properties. Since the MICP process in natural soils is likely to be impacted by the interactions between Sporosarcina pasteurii and indigenous bacteria, we herein examine these interactions and their effects on MICP. The soil extract was used as a source of natural microorganisms, and a control treatment was inoculated in an artificial solution, based on the soil extract with no microorganisms. The following parameters were monitored in the whole process of the experiments: optical density (OD), pH, dissolved Ca2+ and dissolved inorganic carbon (DIC). The results show that dissolved Ca2+ precipitate as CaCO3 more quickly in the control than in natural soil extract. S. pasteurii exhibits a significantly higher growth rate in the artificial soil extract than in the natural solution, which results in a higher density of bacterial cells. We suggest that the presence of the indigenous bacteria decelerates the MICP process by competing with S. pasteurii for nutrients.


Soil improvement Microbial-induced calcite precipitation (MICP) Bio-cementation Sediment 


Funding information

The research work described in this paper was supported by the National Natural Science Foundation of PR China (No. 51809139 and No. 51578293).


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Copyright information

© Saudi Society for Geosciences 2019

Authors and Affiliations

  1. 1.School of Civil EngineeringNanjing Forestry UniversityNanjingPeople’s Republic of China
  2. 2.Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological RestorationNanjing Forestry UniversityNanjingPeople’s Republic of China

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