Biological treatment of red bronze dye through anaerobic process

  • Saima FazalEmail author
  • Shaobin HuangEmail author
  • Yongqing Zhang
  • Zahid Ullah
  • Arshad Ali
  • Hao Xu
Original Paper


Industries that are ever increasing are major contributors to environmental pollution due to inappropriate waste handling. Textile wastewater contains mutagenic and carcinogenic chemicals which are harmful to natural phenomenon of receiving water channels. Therefore, textile wastes should be treated before discharging in the environment. Treatment of red bronze dye was investigated in the upflow anaerobic bioreactor. Varying dye concentrations (40~325 mg/L) were treated by adjusting primary pH at neutral. The performance of reactor was judged by conventional process parameters like pH, turbidity, dissolved oxygen (DO), electroconductivity (EC), total dissolve solids (TDS), removal efficiency, and chemical oxygen demand (COD). The maximum removal efficiency occurred at 150 mg/L dye concentration with addition of 50 ml of biomass. The pH of the water remained basic, COD removal ranged between 60 and 91%, and the maximum removal was done at 91%. EC and TDS were also low indicating good dye removal efficiency of the reactor.


Red bronze dye COD UASB pH EC TDS 


Funding information

This project is financially supported by the National Natural Science Foundation of China (U1701243 and 51572089), the Research Project of Guangdong Provincial Department of Science and Technology (2016B020240002 and 2017A090905029), and the opening foundation of the Jiangsu Key Laboratory of Vehicle Emissions Control (NO.OVEC 044).


  1. Amaral FM, Kato MT, Florêncio L, Gavazza S (2014) Color, organic matter and sulfate removal from textile effluents by anaerobic and aerobic processes. Bioresour Technol 163:364–369CrossRefGoogle Scholar
  2. Babu BR, Parande AK, Kumar SA, Bhanu SU (2011) Treatment of dye effluent by electrochemical and biological processes. Open J Saf Sci Technol 1:12–18CrossRefGoogle Scholar
  3. Barathi S, Indra AP (2015) Decolorization, degradation, and toxicological analysis of textile dye effluent by using novel techniques – review. Int J Sci Res Manage 3(2):2118–2136Google Scholar
  4. Couras CS, Louros VL, Grilo AM, Leitão JH, Capela MI, Arroja LM, Nadais MH (2014) Effects of operational shocks on key microbial populations for biogas production in UASB (upflow anaerobic sludge blanket) reactors. Energy 73:866–874CrossRefGoogle Scholar
  5. da Silva MER, Firmino PIM, dosSantos AB (2013) Reductive decolourisation of sulphonated mono and diazo dyes in one-and two-stage anaerobic systems. Appl Biochem Biotechnol 170:1–14CrossRefGoogle Scholar
  6. De Jager D, Sheldon MS, Edwards W (2014) Colour removal from textile wastewater using a pilot-scale dual-stage MBR and subsequent RO system. Sep Purif Technol 135:135–144CrossRefGoogle Scholar
  7. Gnanapragasam G, Senthilkumar M, Arutchelvan V, Sivarajan P, Nagarajan S (2010) Recycle in upflow anaerobic sludge blanket reactor on treatment of real textile dye effluent. World J Microbiol Biotechnol 26(6):1093–1098CrossRefGoogle Scholar
  8. Gong XB (2016) Advanced treatment of textile dyeing wastewater through the combination of moving bed biofilm reactors and ozonation. Sep Sci Technol 51(9):1589–1597Google Scholar
  9. Haidera A, Khana SJ, Nawazb MS, Saleema MU (2018) Effect of intermittent operation of lab-scale upflow anaerobic sludge blanket (UASB) reactor on textile wastewater treatment. Desalin Water Treat 136:120–130CrossRefGoogle Scholar
  10. Hussain G, Abass N, Shabir G, Athar M, Saeed A, Saleem R, Ali F, Khan MA (2017) New acid dyes and their metal complexes based on substituted phenols for leather: synthesis, characterization and optical studies. J Appl Res Technol 15(4):346–355CrossRefGoogle Scholar
  11. Inthorn D, Singhtho S, Thiravetyan P, Khan E (2004) Decolorization of basic, direct and reactive dyes by pre-treated narrow-leaved cattail (Typha angustifolia Linn.). Bioresour Technol 94(3):299–306CrossRefGoogle Scholar
  12. Lu X, Liu R (2010) Treatment of azo dye-containing wastewater using integrated processes. In: Biodegradation of azo dyes. Springer, Berlin, pp 133–155CrossRefGoogle Scholar
  13. Muda K, Aris A, Salim MR, Ibrahim Z (2013) Sequential anaerobic-aerobic phase strategy using microbial granular sludge for textile wastewater treatment. In: Biomass now-sustainable growth and use IntechOpenGoogle Scholar
  14. Ong SA, Toorisaka E, Hirata M, Hano T (2005) Biodegradation of redox dye methylene blue by upflow anaerobic sludge blanket reactor. J Hazard Mater 124(1-3):88–94CrossRefGoogle Scholar
  15. Palani VR, Rajakumar S, Ayyasamy PM (2012) Exploration of promising dye decolorizing bacterial strains obtained from Erode and Tiruppur textile wastes. Int J Environ Sci 2(4):2470–2481Google Scholar
  16. Pandey A, Singh P, Iyengar L (2007) Bacterial decolorization and degradation of azo dyes. Int Biodeterior Biodegradation 59(2):73–84CrossRefGoogle Scholar
  17. Sarayu K, Sandhya S (2012) Current technologies for biological treatment of textile wastewater–a review. Appl Microbiol Biotechnol 167(3):645–661Google Scholar
  18. Senthilkumar M, Gnanapragasam G, Arutchelvan V, Nagarajan S (2011) Treatment of textile dyeing wastewater using two-phase pilot plant UASB reactor with sago wastewater as co-substrate. Chem Eng J 166(1):10–14CrossRefGoogle Scholar
  19. Tahir U, Yasmin A, Khan UH (2016) Phytoremediation: potential flora for synthetic dyestuff metabolism. J King Saud Uni-Sci 28(2):119–130CrossRefGoogle Scholar
  20. Venkatesh S, Venkatesh K, Quaff AR (2017) Dye decomposition by combined ozonation and anaerobic treatment: cost effective technology. J Appl Res Technol 15(4):340–345CrossRefGoogle Scholar
  21. Waghmode TR, Kurade MB, Kabra AN, Govindwar SP (2012) Biodegradation of Rubine GFL by Galactomyces geotrichum MTCC 1360 and subsequent toxicological analysis by using cytotoxicity, genotoxicity and oxidative stress studies. Microbiology 158(9):2344–2352CrossRefGoogle Scholar
  22. Zhao X, Xu H, Shen J, Yu B, Wang X (2016) Decreasing effect and mechanism of moisture content of sludge biomass by granulation process. Environ Technol 37(2):192–201CrossRefGoogle Scholar

Copyright information

© Saudi Society for Geosciences 2019

Authors and Affiliations

  1. 1.School of Environment and EnergySouth China University of Science and TechnologyGuangzhouPeople’s Republic of China
  2. 2.Guangdong Ecological Environment Control Engineering Technology Research CenterGuangzhouPeople’s Republic of China
  3. 3.Department of Environmental SciencesAllama Iqbal Open UniversityIslamabadPakistan
  4. 4.Military College of Engineering (MCE)National University of Sciences and Technology (NUST) Risalpur CampusRisalpurPakistan

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