Sugar Tech

pp 1–6 | Cite as

Effect of Temperature, Humidity and Light Intensity on Micropropagated Sugarcane (Saccharum Species Hybrid) Genotypes

  • Suresh YadavEmail author
  • T. E. Nagaraja
  • H. C. Lohithaswa
  • K. V. Shivakumar
Research Article


Sugarcane (Saccharum species hybrid) is an important cash crop and is the major source of sugar worldwide. Very little attempt has been made on optimization of conditions like temperature, humidity and light intensity for acclimatization of regenerated plantlets through micropropagation for rapid and high multiplication of disease-free planting material. In this regard, we have carried out an experiment with an objective to know the effect of temperature, light intensity and humidity on hardening of 16 elite sugarcane genotypes under three different hardening conditions, viz. single shade net, double shade net and polyhouse conditions. The single shade net condition was found optimum for shoot morphological and root characteristics in 13 tested sugarcane genotypes. However, the double shade net condition was found optimum, in genotypes VCF 0517, CoVC 07-06-05 and CoVC 07-34-05. The average temperature 28.3 °C, relative humidity 63.20% and light intensity (7522 lx) found to be best for 13 sugarcane genotypes for their shoot morphological and root characteristics under singe shade net within a short period of time.


Sugarcane Micropropagation Temperature Hardening Mortality 


Supplementary material

12355_2019_779_MOESM1_ESM.docx (5.2 mb)
Supplementary material 1 (DOCX 5308 kb)


  1. Arun, K.T., and C.T. Baishnab. 1998. Temperature stress induced impairment of chlorophyll biosynthetic reactions in cucumber and wheat. Plant Physiology 117: 851–858.CrossRefGoogle Scholar
  2. Debergh, P.C., and L. Maene. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Scientia Horticulturae 14: 335–345.CrossRefGoogle Scholar
  3. Deng, R., and F.D. Donnelly. 1993. ln vitro hardening of red raspberry through CO2 enrichment and relative humidity reduction on sugar-free medium. Canadian Journal of Plant Science 73: 1105–1113.CrossRefGoogle Scholar
  4. Grout, B.W.W., and M.J. Aston. 1977. Transplanting of cauliflower plants regenerated from meristem culture: I. Water loss and water transfer related to changes in leaf wax and to xylem regeneration. Horticulture Research 17: 1–7.Google Scholar
  5. Horn, W., G. Schlegel, and K. John. 1988. Micropropagation of roses (Rosa hybr.). Acta Horticulturae 226: 623–626.CrossRefGoogle Scholar
  6. Kumar, K., and I.U. Rao. 2012. Morpho-physiologicals problems in acclimatization of micropropagated plants in ex vitro conditions—A review. Journal of Horticultural Science and Ornamental Plants 2 (4): 271–283.Google Scholar
  7. Lal, N., and R.G. Singh. 1994. Rapid clonal multiplication of sugarcane through tissue culture. Plant Tissue Culture 4: 1–7.Google Scholar
  8. Lal, M., A.K. Tiwari, G.N. Gupta, and Kavita. 2014. Commercial scale micropropagation of sugarcane: Constraints and remedies. Sugar Tech. 17: 339–347.CrossRefGoogle Scholar
  9. Murashige, T. 1974. Plant propagation through tissue cultures. Annual Review of Plant Physiology 25: 135–166.CrossRefGoogle Scholar
  10. Pospisilova, J., H. Synkova, D. Haisel, and S. Semoradova. 2007. Acclimation of plantlets to ex vitro conditions: Effects of air humidity, irradiance, CO2 concentration and abscisic acid (a review). Acta Horticulturae 748: 29–38.CrossRefGoogle Scholar
  11. Pospisilova, J., I. Ticha, P. Kadlecek, D. Haisel, and S. Plzakova. 1999. Acclimatization of micropropagated plants to ex vitro conditions. Biologia Plantarum 42 (4): 481–497.CrossRefGoogle Scholar
  12. Rani, A. and S. Kumar. 2017. Tissue culture as a plant production technique for medicinal plants: A review. In International Conference on Innovative Research in Science Technology and Management, 22nd–23rd January 2017, 609–620. Dadari, Kota, Rajasthan.Google Scholar
  13. Schmauder, H.P., E.F. George, and P.D. Sherrington. 1985. Plant propagation by tissue culture — handbook and directory of commercial laboratories. Journal of Basic Microbiology 25 (7): 475–475.CrossRefGoogle Scholar
  14. Shailesh, R.V., and R.M. Kothan. 2007. An integrated approach to primary and secondary hardening of banana variety Grand Naine. Indian Journal of Biotechnology 7: 240–245.Google Scholar
  15. Short, K.C., J. Warburton, and A.V. Robert. 1987. In vitro hardening of cultured cauliflower and chrysanthemum plantlets to humidity. Acta Horticulturae 212: 329–334.CrossRefGoogle Scholar
  16. Telles, C.A., and L.A. Biasi. 2005. Rooting in vitro and acclimatization in greenhouse of Japanese persimmon (Diospyrus kaki L.). Ciencia E Agrotecnologia 29 (2): 481–484.CrossRefGoogle Scholar
  17. Wrigley, G. 1982. Tropical agriculture: The development of production, 4th ed. New York: Longman Inc.Google Scholar
  18. Ziv, M. 1986. In vitro hardening and acclimatization of tissue cultured plantlets. In Plant tissue culture and items agricultural applications, ed. L.A. Withers and P.G. Alderson, 187–196. London: Butterworths.CrossRefGoogle Scholar

Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  1. 1.Indian Agricultural Research InstituteNew DelhiIndia
  2. 2.Department of Genetics and Plant Breeding, College of Agriculture, V.C. Farm, MandyaUniversity of Agricultural Sciences, BengaluruMandya 571405India

Personalised recommendations