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Sugar Tech

, Volume 21, Issue 6, pp 909–916 | Cite as

Characteristics, Expression Pattern and Intracellular Localisation of Sugarcane Cytoplasmic Hexokinase Gene ShHXK8

  • Jun-gang Wang
  • Ting-ting Zhao
  • Ben-peng Yang
  • Wen-zhi Wang
  • Cui-lian Feng
  • Xiao-yan Feng
  • Lin-bo Shen
  • Shu-zhen ZhangEmail author
Research Article
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Abstract

Hexokinase (HXK) catalyses hexose phosphorylation and senses glucose signalling to control carbon partition in plants. In sugarcane, HXK is involved in regulating source/sink carbon balance. However, the characteristics of sugarcane HXK genes remain unclear. This study revealed that the full-length cDNA sequence of the HXK gene ShHXK8 cloned from sugarcane mature leaves contains a 1560 bp open reading frame and encodes a putative protein of 519 amino acids. Amongst HXKs from monocot and dicot subjected to phylogenetic analysis, ShHXK8 was clustered into a monocot-specific group with OsHXK7, SbHXK8 and ZmHXK1. Gene expression analysis in Saccharum spp. hybrid ROC22 plants showed that ShHXK8 was expressed in leaves and stalks and expressed highly in mature leaves and young internodes. The difference in the expression of ShHXK8 was compared in high-sugar-content (HSC) and low-sugar-content (LSC) Saccharum officinarum plants. The expression of ShHXK8 in the leaves and internodes 3–24 of HSC plants was lower than that of LSC plants. The expression of ShHXK8 in the internodes 1–2 of HSC plants was higher than that of LSC plants. ShHXK8–GFP fusion was located in the cytosol by transiently expressing the corresponding vectors in rice mesophyll protoplasts. ShHXK8 might function as a cytoplasmic hexokinase involved in hexose metabolism in sugarcane mature leaves and young internodes.

Keywords

Sugarcane Hexokinase ShHXK8 Subcellular location 

Abbreviations

FRK

Fructokinase

F6P

Fructose-6-phosphate

G6P

Glucose-6-phosphate

HSC

High sugar content

HXK

Hexokinase

LSC

Low sugar content

ORF

Open reading frame

PCR

Polymerase chain reaction

RT

Reverse transcription

UDP-G

UDP-glucose

Notes

Acknowledgements

This research was supported by Grants from the National Key Research and Development Program of China (2018YFD1000503), Sugar Crop Research System (CARS-170301), Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences (No. 1630052019028) and the Program of Hainan Association for Science and Technology Plans to Youth R & D Innovation (HAST201626).

Author Contributions

SZZ conceived and designed the experiments. JW and TZ performed the experiments, result analysis, and manuscript drafting. BY, WW, CF, XF and LS revised the manuscript. All authors have read and approved the final manuscript.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  • Jun-gang Wang
    • 1
  • Ting-ting Zhao
    • 1
  • Ben-peng Yang
    • 1
  • Wen-zhi Wang
    • 1
  • Cui-lian Feng
    • 1
  • Xiao-yan Feng
    • 1
  • Lin-bo Shen
    • 1
  • Shu-zhen Zhang
    • 1
    Email author
  1. 1.Institute of Tropical Bioscience and Biotechnology of Chinese Academy of Tropical Agricultural Sciences, Sugarcane Research Center of Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of Biology and Genetic Resources of Tropical CropsMinistry of AgricultureHaikouChina

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