Sugar Tech

, Volume 21, Issue 6, pp 891–897 | Cite as

Molecular Cloning and Expression Analysis of Ethylene-Insensitive3-Like 1 (ScEIL1) Gene in Sugarcane

  • Fan-Wei Wang
  • Jian-Bin Yu
  • Dong Xiao
  • Yang-Rui LiEmail author
  • Long-Fei He
  • Ai-Qin Wang
Research Article


Ethylene-insensitive3-like 1 (EIL1) is a transcription factor involving in ethylene signal transduction pathway. In the present study, a ScEIL1 gene encoding EIL1 was cloned from a sugarcane cv. GT 28 (Saccharum hybrid cultivar) using RT-PCR with primers designed based on conserved amino acid sequences of EIL1 from corn and sorghum. The ScEIL1 gene had 2179 base pairs in length and coded for a polypeptide of 639 amino acid residues. The molecular mass of deduced sugarcane EIL1 protein was 71.2 kDa, and the pI was 5.05. The amino acid sequence of ScEIL1 encoded protein was 97, 92 and 91% identical to SbEIL1 (XP 002467935.1) of sorghum, ZmEIL1 (ACG45492.1) of corn and SiEIL1 (XP 004984528.1) of millet, respectively. Real-time PCR analyses results showed ScEIL1 had much higher expression level in the late maturing varieties than in the early maturing varieties, and the expression level of ScEIL1 was higher in the maturing internodes than in the immature and matured internodes, while in leaves it tends to decrease with leaf development and maturation at mature stage. The results from this study may help understand the ethylene regulated sucrose accumulation in sugarcane stalks and leaf development.


Sugarcane Ethylene-insensitive3-like 1 (EIL1) Cloning Gene expression 



This work was supported by the China National R&D Research Program Project (81860670), the Guangxi Special Fund for Scientific Base and Talent (GKAD17195100) and the Guangxi Sugarcane Innovation Team of National Agricultural Industry Technology System (gjnytxgxcxtd-03-01).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.


  1. Alonso, J.M., A.N. Stepanova, R. Solano, E. Wisman, S. Ferrari, F.M. Ausubel, and J.R. Ecker. 2003. Five components of the ethylene-response pathway identified in a screen for weak ethylene-insensitive mutants in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 100 (5): 2992–2997.CrossRefGoogle Scholar
  2. Bailey, S.J., T. Fukao, D.J. Gibbs, M.J. Holdsworth, S.C. Lee, F. Licausi, P. Perata, L.A. Voesenek, and J.T. van Dongen. 2012. Making sense of low oxygen sensing. Trends in Plant Science 17 (3): 129–138.CrossRefGoogle Scholar
  3. Bie, B.B., J.S. Pan, H.L. He, H.L. Yang, H.L. Zhao, and R. Cai. 2013. Molecular cloning and expression analysis of the ethylene insensitive3 (EIN3) gene in cucumber (Cucumis sativus). Genetics and Molecular Research 12 (4): 4179–4191.CrossRefGoogle Scholar
  4. Bleecker, A.B., and H. Kende. 2000. Ethylene: A gaseous signal molecule in plants. Annual Review of Cell and Developmental Biology 16 (1): 1–18.CrossRefGoogle Scholar
  5. Chao, Q., M. Rothenberg, R. Solano, G. Roman, W. Terzaghi, and J.R. Ecker. 1997. Activation of the ethylene gas response pathway in Arabidopsis by the nuclear protein ethylene-insensitive3 and related proteins. Cell 89 (7): 1133–1144.CrossRefGoogle Scholar
  6. Feng, Y., P. Xu, B. Li, P. Li, X. Wen, F. An, Y. Gong, Y. Xin, Z. Zhu, Y. Wang, and H. Guo. 2017. Ethylene promotes root hair growth through coordinated EIN3/EIL1 and RHD6/RSL1 activity in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 114 (52): 13834–13839.CrossRefGoogle Scholar
  7. Gao, S., D.X. Xu, Z.S. Chen, X.X. Xie, K.Q. Zhang, B.Y. Lin, and Q.F. Wen. 2015. Cloning and expression analysis of transcription factor Mc EIL2 in Momordica charantia L. Molecular Plant Breeding 13 (6): 1309–1317.Google Scholar
  8. Guo, H., and J.R. Ecker. 2003. Plant responses to ethylene gas are mediated by SCF(EBF1/EBF2)-dependent proteolysis of EIN3 transcription factor. Cell 115: 667–677.CrossRefGoogle Scholar
  9. Ju, C., G.M. Yoon, J.M. Shemansky, D.Y. Lin, Z.I. Ying, J. Chang, W.M. Garrret, M. Kessenbrock, G. Groth, M.L. Tucker, B. Cooper, J.J. Kieber, and C. Chang. 2012. CTR1 phosphorylates the central regulator EIN2 to control ethylene hormone signaling from the ER membrane to the nucleus in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 109: 19486–19491.CrossRefGoogle Scholar
  10. Li, Y.R., and S. Solomon. 2003. Ethephon: A versatile growth regulator for sugarcane industry. Sugar Tech 5: 213–223.CrossRefGoogle Scholar
  11. Li, Y.R., and L.T. Yang. 2015. Sugarcane agriculture and sugar industry in China. Sugar Tech 17 (1): 1–8.CrossRefGoogle Scholar
  12. Peng, J., Z. Li, X. Wen, W. Li, H. Shi, L. Yang, H. Zhu, and H. Guo. 2014. Salt-induced stabilization of EIN3/EIL1 confers salinity tolerance by deterring ROS accumulation in Arabidopsis. PLoS Genetics 10 (10): e1004664.CrossRefGoogle Scholar
  13. Potuschak, T., E. Lechner, Y. Parmentier, S. Yanagisawa, S. Grava, C. Koncz, and P. Genschik. 2003. EIN3-dependent regulation of plant ethylene hormone signaling by two Arabidopsis F box proteins. Cell 115 (6): 679–689.CrossRefGoogle Scholar
  14. Rafael, G.T., P. Lakshmanan, E. Peiter, A. O’Connell, C. Caldana, R. Vicentini, J.S. Soares, and M. Menossi. 2018. ScGAI is a key regulator of culm development in sugarcane. Journal of Experimental Botany 69 (16): 3823–3837.CrossRefGoogle Scholar
  15. Shi, Q.L., Y.B. Dong, D.H. Qiao, Q. Zhou, L. Zhang, Z.Y. Ma, and Y.L. Li. 2017. Characterization and functional analysis of transcription factor ZmEIL1 in maize. Biologia Plantarum 61 (2): 266–274.CrossRefGoogle Scholar
  16. Solomon, S., H.N. Shahi, S.K.D. Majumder, I. Singh, V.K. Madan, and D.M. Hogarth. 2001. Effect of ethephon on sugarcane grown under subtropical climates. International Society of Sugar Cane Technologists XXIV Congress 2: 174–176.Google Scholar
  17. Su, L., S.H. Wang, Y.J. Hao, X.F. Wang, and C.X. You. 2016. Molecular cloning and functional characterization of the ethylene signaling related gene MdEIL1 in apple. Acta Horticulturae Sinica 43 (3): 409–418.Google Scholar
  18. Tamura, K., G. Stecher, D. Peterson, A. Filipski, and S. Kumar. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution 30: 2725–2729.CrossRefGoogle Scholar
  19. Wang, A.Q., Y.G. Fan, X.Y. Zhao, L.F. He, L.T. Yang, and Y.R. Li. 2008. Expressions of three members of ACS gene family induced by ethephon relationship of ethylene production and sugar accumulation in sugarcane stalks. Acta Agronomica Sinica 34: 418–422.CrossRefGoogle Scholar
  20. Wang, A.Q., W.J. Huang, J.Q. Niu, M. Liu, L.T. Yang, and Y.R. Li. 2013. Effects of ethephon on key enzymes of sucrose metabolism in relation to sucrose accumulation in sugarcane. Sugar Tech 15 (2): 177–186.CrossRefGoogle Scholar
  21. Wang, A.Q., L.T. Yang, Z.Z. Wang, Y.T. Wei, L.F. He, and Y.R. Li. 2006. Expression of three members of ACC synthase gene family in sugarcane induced by hormones and environmental stress. Acta Agronomica Sinica 32: 734–737.Google Scholar
  22. Wang, A.Q., X.Z. Ye, J.L. Huang, J.Q. Niu, M. Liu, Y.B. Pan, L.T. Yang, and Y.R. Li. 2015. Molecular cloning and functional analysis of an ethylene receptor gene from sugarcane (Saccharum spp.) by hormone and environmental stresses. Sugar Tech 17 (1): 22–30.CrossRefGoogle Scholar
  23. Zhang, F., L. Wang, E.E. Ko, K. Shao, and H. Qiao. 2018. Histone deacetylases SRT1 and SRT2 interact with ENAP1 to mediate ethylene-induced transcriptional repression. The Plant Cell 30 (1): 153–166.CrossRefGoogle Scholar
  24. Zhu, Z., F. An, Y. Feng, P. Li, X. Li, A. Mu, Z. Jiang, J.M. Kim, T. Kim, W. Li, X. Zhang, Q. Yu, Z. Dong, W.Q. Chen, M. Seki, J.M. Zhou, and H. Guo. 2011. Derepression of ethylene-stabilized transcription factors (EIN 3/EIL1) ediates jasm onate and ethylene signaling synergy in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America 108 (30): 12539–12544.CrossRefGoogle Scholar

Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  1. 1.College of AgricultureGuangxi UniversityNanningChina
  2. 2.Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Guangxi Key Laboratory of Sugarcane Genetic ImprovementGuangxi Academy of Agricultural SciencesNanningChina

Personalised recommendations