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Sugar Tech

, Volume 21, Issue 6, pp 936–948 | Cite as

Effects of Exogenous GA3 and DPC Treatments on Levels of Endogenous Hormone and Expression of Key Gibberellin Biosynthesis Pathway Genes During Stem Elongation in Sugarcane

  • Li-Hang Qiu
  • Rong-Fa Chen
  • Han-Min Luo
  • Ye-Geng Fan
  • Xing Huang
  • Jun-Xian Liu
  • Fa-Qian XiongEmail author
  • Hui-Wen Zhou
  • Chong-Kun Gan
  • Jian-Ming WuEmail author
  • Yang-Rui LiEmail author
Research Article
  • 68 Downloads

Abstract

Internode elongation determines plant height, which in turn affects the cane yield in sugarcane. This study aimed to explore the mechanisms of sugarcane internode elongation regulation by plant growth regulators, gibberellic acid (GA3) and its inhibitor 1, 1-dimethylpiperidinium chloride (DPC, also called mepiquat chloride). We sprayed GA3 or DPC on the leaves at the early shoot elongation stage with water as the control. The contents of endogenous hormones and the expression of GA20 ox1, GID1, and GAI in sugarcane leaves were measured at days 3, 6, 12, and 24 after treatment. It was found that the height of sugarcane plants treated with GA3 showed a significant increase compared to the control, whereas an opposite trend was observed in the DPC-treated plants. Compared to the control, the content of gibberellins (GAs) in leaves was increased significantly, while the indole acetic acid (IAA) content showed an initial increase, then a decline, and eventually remained stable in the GA3-treated plants. The contents of cytokinins (CTK), abscisic acid (ABA), and ethylene (ETH) in leaves exhibited a significant downward trend in the GA3-treated plants compared to the control. In the DCP-treated plants, however, the levels of ABA and ETH in leaves were significantly increased, IAA and GAs significantly decreased, and CTK showed a trend of an initial decline followed by an increase. After GA3 treatment, the gene expression levels of GA20 ox1 and GID1 in sugarcane increased first then decreased, whereas the gene expression level of GAI first decreased and then stabilized. After DPC treatment, the expression of GA20 ox1 in sugarcane showed an initial decrease followed by an increase, while the expression of GID1 decreased gradually and then stabilized, and the expression of GAI showed a trend of an initial increase followed by stabilization. The results of the present study indicate that exogenous GA3 treatment stimulated the internode elongation in sugarcane mainly by increasing the endogenous levels of GAs, decreasing the levels of ABA and ETH, and regulating the expression of GA20 ox1, GID1 and GAI genes; the DPC treatment mainly increased the contents of ABA and ETH and reduced the contents of GAs and IAA, thereby inhibiting the expression of GID1, promoting the expression of GAI, and ultimately inhibiting the internode elongation in sugarcane.

Keywords

GA Mepiquat chloride Sugarcane Internode elongation Endogenous hormone Gene expression Regulation 

Notes

Acknowledgements

The authors are grateful to Dr. Prakash Lakshmanan for his critical revision of this manuscript.

Funding

National Natural Science Foundation of China (31701363, 31360312 and 31660356), Project for Guangxi Sugarcane Innovation Team of National Modern Agricultural History Technology System (nycytxgxcxtd-03-01), Guangxi Natural Science Foundation (2015GXNSFBA139011, 2016GXNSFBA380034, 2017GXNSFBA198050, and 2018GXNSFAA138149), Guangxi Key Laboratory Construction Project (15-140-13), Guangxi Science and Technology Project (GKG 1598006-1-2E), and Guangxi Academy of Agricultural Sciences Fund (2017YM03, 2018YT01, and 2018YM02).

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  1. 1.Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences-Sugarcane Research CenterChinese Academy of Agricultural SciencesNanningChina
  2. 2.Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of AgricultureP.R. China-Guangxi Key Laboratory of Sugarcane Genetic ImprovementNanningChina
  3. 3.Soil and Fertilizer Station of Chongzuo CityChongzuoChina

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