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Phenological Changes in the Chlorophyll Content and Its Fluorescence in Field-Grown Sugarcane Clones Under Over-Wintering Conditions

  • Takeo SakaigaichiEmail author
  • Hiroka Tsuchida
  • Katsuki Adachi
  • Taiichiro Hattori
  • Yusuke Tarumoto
  • Minoru Tanaka
  • Michiko Hayano
  • Jun-Ichi Sakagami
  • Shin Irei
Short Communication
  • 14 Downloads

Abstract

As a step toward breeding cultivars with tolerance to chilling stress, phenological changes in chlorophyll content (SPAD index) and its fluorescence (Fv/Fm) were investigated under over-wintering conditions using different sugarcane clones. A leading commercial cultivar in Japan, NiF8, and two interspecific lines, KY11-6114 and KY08-1200, were tested in this study. Daily minimum temperature decreased gradually with the advance of winter, and the lowest temperature of 1.2 °C was recorded in January. Although the temperature increased toward spring season, it remained below 10 °C until the end of experiment. Stem elongations in KY11-6114 and KY08-1200 during winter were significantly greater than in NiF8. The decrease in SPAD index was calculated from the difference between the beginning of winter and each measuring time. KY08-1200 and KY11-6114, with longer stem elongation, had a smaller decrease in SPAD index than NiF8 under over-wintering conditions. The Fv/Fm of all three clones decreased in January when the lowest temperature was recorded and recovered toward spring. Among three clones, KY08-1200 was less affected by chilling stress and presented a higher value of Fv/Fm up to the end of experiment. Considering these results, we concluded that the decreased chlorophyll content measured by the SPAD index was a simple indicator for screening chilling-tolerant clones.

Keywords

Sugarcane Chilling stress Chlorophyll content Chlorophyll fluorescence Over-wintering 

Notes

Acknowledgements

A part of this research was supported by a grant from Okinawa Prefecture. The authors appreciate technical supports received from Mr. Kubo, M., Mr. Oitate, Y., Mr. Habu, M., and Mr. Yano, S. of the NARO/KARC Tanegashima sugarcane breeding site. We would like to thank Editage for English language editing.

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Copyright information

© Society for Sugar Research & Promotion 2019

Authors and Affiliations

  • Takeo Sakaigaichi
    • 1
    • 4
    Email author
  • Hiroka Tsuchida
    • 1
  • Katsuki Adachi
    • 1
  • Taiichiro Hattori
    • 1
  • Yusuke Tarumoto
    • 1
  • Minoru Tanaka
    • 1
  • Michiko Hayano
    • 1
  • Jun-Ichi Sakagami
    • 2
  • Shin Irei
    • 3
  1. 1.NARO Kyushu Okinawa Agricultural Research CenterNishinoomoteJapan
  2. 2.Kagoshima UniversityKagoshimaJapan
  3. 3.Okinawa Prefectural Agricultural Research CenterItomanJapan
  4. 4.NARO Kyushu Okinawa Agricultural Research CenterMiyakonojoJapan

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