Phenological Changes in the Chlorophyll Content and Its Fluorescence in Field-Grown Sugarcane Clones Under Over-Wintering Conditions
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As a step toward breeding cultivars with tolerance to chilling stress, phenological changes in chlorophyll content (SPAD index) and its fluorescence (Fv/Fm) were investigated under over-wintering conditions using different sugarcane clones. A leading commercial cultivar in Japan, NiF8, and two interspecific lines, KY11-6114 and KY08-1200, were tested in this study. Daily minimum temperature decreased gradually with the advance of winter, and the lowest temperature of 1.2 °C was recorded in January. Although the temperature increased toward spring season, it remained below 10 °C until the end of experiment. Stem elongations in KY11-6114 and KY08-1200 during winter were significantly greater than in NiF8. The decrease in SPAD index was calculated from the difference between the beginning of winter and each measuring time. KY08-1200 and KY11-6114, with longer stem elongation, had a smaller decrease in SPAD index than NiF8 under over-wintering conditions. The Fv/Fm of all three clones decreased in January when the lowest temperature was recorded and recovered toward spring. Among three clones, KY08-1200 was less affected by chilling stress and presented a higher value of Fv/Fm up to the end of experiment. Considering these results, we concluded that the decreased chlorophyll content measured by the SPAD index was a simple indicator for screening chilling-tolerant clones.
KeywordsSugarcane Chilling stress Chlorophyll content Chlorophyll fluorescence Over-wintering
A part of this research was supported by a grant from Okinawa Prefecture. The authors appreciate technical supports received from Mr. Kubo, M., Mr. Oitate, Y., Mr. Habu, M., and Mr. Yano, S. of the NARO/KARC Tanegashima sugarcane breeding site. We would like to thank Editage for English language editing.
- Du, Y.C., A. Nose, and K. Wasano. 1999. Effect of chilling temperature on photosynthetic rates, photosynthetic enzyme activities and metabolite levels in leaves of three sugarcane species. Plant and Cell Physiology 22: 317–324.Google Scholar
- Irvine, J.E. 1978. Identification of cold tolerance in Saccharum and related genera through refrigerated freeze screening. Proceedings of the International Society of Sugar Cane Technologists 16: 147–156.Google Scholar
- Roach, B.T. 1978. Utilization of Saccharum spontaneum in sugarcane breeding. Proceedings of the International Society of Sugar Cane Technologists 16: 43–57.Google Scholar
- Sakaigaichi, T., Y. Terajima, M. Matsuoka, T. Terauchi, I. Hattori, T. Suzuki, A. Sugimoto, and T. Hattori. 2010. The growth and yield of the ratoon crop of forage sugarcane variety, KRFo93-1, in twice harvesting system. Japanese Journal of Crop Science 79: 414–423. (in Japanese with English abstract).CrossRefGoogle Scholar
- Tang, S.Y., Y.R. Li, and L.T. Yang. 2015. Evaluation of cold tolerance and photosynthetic characteristics in different sugarcane genotypes. Journal of Global Biosciences 4: 2459–2467.Google Scholar