Agrobacterium rhizogenes-Mediated Transformation Enhances Steviol Glycosides Production and Growth in Stevia rebaudiana Plantlets
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Stevia rebaudiana accumulates steviol glycosides (SGs) that are used as noncaloric, natural sweeteners in food industry. The most important SGs are rebaudioside A (Reb A) and stevioside (St), and they are markers of the quality of S. rebaudiana lines. In this work, the production and physiology of SGs in Agrobacterium rhizogenes-transformed plantlets were analyzed. By means of HPLC–DAD, the production of St and Reb A was quantified, resulting in a 1.4- and 1.5-fold production increase of St and Reb A, respectively, in transformed compared to wild-type plantlets. Superior phenotype of transformed plantlets was observed in comparison with wild type, represented as an increase of 25% in biomass of aerial parts, 43% in biomass of roots, 20–30% of leaf area and 24% of chlorophylls content. Moreover, the SG production profiles evaluated in a 20-d period showed higher yield in the transformed line. The results of this work demonstrated the usefulness of S. rebaudiana transformation via A. rhizogenes as a strategy to explore new approaches for the improvement of SGs production.
KeywordsStevia rebaudiana Stevioside Genetic transformation Rebaudioside A Agrobacterium
The authors are grateful to the National Council of Science and Technology (Consejo Nacional de Ciencia y Tecnología—CONACyT) for scholarship No. 615648 to AJSC and to Catedras-CONACyT 3212 (235307) and INFR201501 (255514).
A.J.S-C carried out laboratory work and data analysis. J.C-T contributed to the equipment and B.E.B-F edited the manuscript. P.M.S-O. and A.L-T. contributed to the data interpretation and statistical analysis. A.A.H-H. and E.G-L. designed and analyzed the experiments and wrote the manuscript. All authors reviewed and approved the final manuscript.
Compliance with Ethical Standards
Conflict of interest
The authors declare that they have no conflict of interest.
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