Nandrolone decanoate in induced fracture nonunion with vascular deficit in rat model: morphological aspects
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The nonunion fracture is a relatively frequent complication in both human and veterinary medicine. Specifically, atrophic fracture nonunions are difficult to treat, with revision surgery usually providing the best prognosis. Anabolic steroids, such as nandrolone decanoate (ND), have been reported to have beneficial clinical effects on bone mass gain during osteoporosis; however, their utility in promoting regeneration in atrophic nonunions has not been documented. Our objective was to examine morphological changes induced by the ND in experimental fracture nonunion with vascular deficit in the rat model.
Fourteen adult Wistar rats had an atrophic fracture nonunion induced in the diaphysis of their left femur. Rats were allocated into two groups: control group and nandrolone decanoate group. Rats in the latter group were given nandrolone decanoate (1.5 mg/kg IM, once a week, during 4 weeks after confirmation of fracture nonunion radiographically). Radiographic and anatomopathological examination, micro-tomography and histological analysis were assessed to characterize the morphological changes promoted by the nandrolone decanoate use.
Based on radiology, anatomopathological evaluation, computed micro-tomography and conventional microscopy, nandrolone decanoate promoted bone regeneration at the fracture nonunion site by increasing the cellularity at the fracture site. Percentage of collagen was not significantly different between groups, consistent with high-quality regenerated bone.
The anabolic steroid nandrolone decanoate improved bone mass and regeneration without affecting collagen production and therefore has potential for improving outcomes for atrophic fracture nonunion.
KeywordsAndrogenic Atrophic Bone Healing Regeneration Steroid
We thank Professor Dr Kurt Hankenson from University of Michigan, Professor Dr John Kastelic from University of Calgary and Professor Dr Concepta Margaret McManus Pimentel from Universidade Federal do Rio Grande do Sul for revising this manuscript. This study was partly funded by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) of the Brazilian Government doctorate scholarship program.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- 2.Mills LA, Hamish A, Simpson RW (2013) The relative incidence of fracture non-union in the Scottish population (5.17 million): a 5-year epidemiological study. BMJ 3(2):002276Google Scholar
- 4.Rubin E, Farber JL (2002) Patologia. Editora Guanabara Koogan Ltda, Rio de JaneiroGoogle Scholar
- 5.Piermattei DL, Flo GL, DeCamp CE (2009) Brinker, Piermattei, Flo: Ortopedia e Tratamento de Fraturas de Pequenos Animais. Manole, BarueriGoogle Scholar
- 7.Heckman JD, Sarasohn-Kahn J (1997) The economics of treating tibia fractures: the cost of delayed unions. Bull Hosp Jt Dis 56(1):63–72Google Scholar
- 16.Aerssens J, Audekerecke RV, Geusens P, Schot LP, Osman AA (1993) Mechanical properties, bone mineral content, and bone composition (collagen, osteocalcin, IGF-I) of the rat femur: influence of ovarectomy and nandrolone decanoate (anabolic steroid). Calcif Tissue Int 53(4):360–366CrossRefGoogle Scholar
- 18.Ahmad F, Yunus SN, Asghar A, Faruqi NA (2013) Influence of anabolic steroid on tibial fracture healing in rabbits—a study on experimental model. J Clin Diagn Res 7(1):93–96Google Scholar
- 24.Geusens P, Dequeker J (1986) Long-term effect of nandrolone decanoate, 1α hydroxyvitamin D3 or intermittent calcium infusion therapy on bone mineral content, bone remodeling and fracture rat in sympomatic osteoporotic: a double-blind controled study. Bone Miner 1(4):347–357Google Scholar