Physiology and Molecular Biology of Plants

, Volume 25, Issue 5, pp 1235–1249 | Cite as

Exploring of greater yam (Dioscorea alata L.) genotypes through biochemical screening for better cultivation in south Gujarat zone of India

  • Kajal S. Patel
  • Nilima KarmakarEmail author
  • Ketan D. Desai
  • Ajay V. Narwade
  • Gayacharan Chakravarty
  • Manoj Kanti Debnath
Research Article


Fifteen different genotypes of greater yam (Dioscorea alata) NGY-1, NGY-2, NGY-3, NGY-4, NGY-5, NGY-6, NGY-7, NGY-8, NGY-9, NGY-10, NGY-11, NGY-12, NGY-13, NGY-14 and Da-199 were procured from different places of south Gujarat, like Valsad, Navsari, Surat and Anand. Among the biochemical parameters total carbohydrate ranged between 51.87 and 87.85% from different genotypes, starch ranged from 47 to 80.67%, crude fat ranged from 0.6 to 2.32%, crude fibre ranged between 1.10 and 4.09%, anthocyanin content of genotypes ranged from 1.01 to 3.25 mg/g, beta-carotene content ranged between 0.97 and 1.88 µg/g. The antinutrients namely Diosgenin and Tannin ranged from 0.28 to 0.93% and 0.923 mg/100 g to 2.447 mg/100 g, respectively. RAPD analysis was also done by the help of 18 RAPD markers: OPA1, OPA2, OPA3, OPA13, OPB1, OPB6, OPB7, OPM1, OPM2, OPM4, OPM7, OPM11, OPM12, OPM13, OPM15, OPM16, OPM17 and OPM19 from which an average of 9.7 loci were detected with an average of 4.72 polymorphic loci which is 48.65% polymorphism per loci and 48.70% average polymorphism. From the overall study, it can be conferred that the study revealed highest carbohydrate content in NGY-3 (87.85%), fat (2.32%) and crude fiber content (4.09%) in NGY-11, β-carotene in NGY-7 (1.88 µg/g), anthocyanin in NGY-4(3.25 mg/g). Lowest tannin (0.923 mg/100 g) and diosgenin (0.28%) was found in NGY-6, and NGY-7 respectively. For each of the biochemical parameters, the varieties with the optimum values may be cultivated. As the molecular studies revealed NGY-2 and NGY-3 have 96% similarities, they may be the duplicate of the same genotypes which can be studied further for better germplasm conservation.


Greater yam Biochemical parameters Antinutrients RAPD 


Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interest.


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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Department of Soil Science and Agricultural Chemistry, N M College of AgricultureNavsari Agricultural UniversityNavsariIndia
  2. 2.ASPEE College of HorticultureNavsari Agricultural UniversityNavsariIndia
  3. 3.Department of Genetics and Plant Breeding, N M College of AgricultureNavsari Agricultural UniversityNavsariIndia
  4. 4.Division of Germplasm EvaluationICAR-NBPGRNew DelhiIndia
  5. 5.Faculty of AgricultureUttar Banga Krishi ViswavidyalayaPundibari, Cooch BeharIndia

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