Advertisement

Physiology and Molecular Biology of Plants

, Volume 25, Issue 5, pp 1235–1249 | Cite as

Exploring of greater yam (Dioscorea alata L.) genotypes through biochemical screening for better cultivation in south Gujarat zone of India

  • Kajal S. Patel
  • Nilima KarmakarEmail author
  • Ketan D. Desai
  • Ajay V. Narwade
  • Gayacharan Chakravarty
  • Manoj Kanti Debnath
Research Article
  • 48 Downloads

Abstract

Fifteen different genotypes of greater yam (Dioscorea alata) NGY-1, NGY-2, NGY-3, NGY-4, NGY-5, NGY-6, NGY-7, NGY-8, NGY-9, NGY-10, NGY-11, NGY-12, NGY-13, NGY-14 and Da-199 were procured from different places of south Gujarat, like Valsad, Navsari, Surat and Anand. Among the biochemical parameters total carbohydrate ranged between 51.87 and 87.85% from different genotypes, starch ranged from 47 to 80.67%, crude fat ranged from 0.6 to 2.32%, crude fibre ranged between 1.10 and 4.09%, anthocyanin content of genotypes ranged from 1.01 to 3.25 mg/g, beta-carotene content ranged between 0.97 and 1.88 µg/g. The antinutrients namely Diosgenin and Tannin ranged from 0.28 to 0.93% and 0.923 mg/100 g to 2.447 mg/100 g, respectively. RAPD analysis was also done by the help of 18 RAPD markers: OPA1, OPA2, OPA3, OPA13, OPB1, OPB6, OPB7, OPM1, OPM2, OPM4, OPM7, OPM11, OPM12, OPM13, OPM15, OPM16, OPM17 and OPM19 from which an average of 9.7 loci were detected with an average of 4.72 polymorphic loci which is 48.65% polymorphism per loci and 48.70% average polymorphism. From the overall study, it can be conferred that the study revealed highest carbohydrate content in NGY-3 (87.85%), fat (2.32%) and crude fiber content (4.09%) in NGY-11, β-carotene in NGY-7 (1.88 µg/g), anthocyanin in NGY-4(3.25 mg/g). Lowest tannin (0.923 mg/100 g) and diosgenin (0.28%) was found in NGY-6, and NGY-7 respectively. For each of the biochemical parameters, the varieties with the optimum values may be cultivated. As the molecular studies revealed NGY-2 and NGY-3 have 96% similarities, they may be the duplicate of the same genotypes which can be studied further for better germplasm conservation.

Keywords

Greater yam Biochemical parameters Antinutrients RAPD 

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they do not have any conflict of interest.

References

  1. Adegunwa MO, Alamu EO, Omitogun LA (2011) Effect of processing on the nutritional contents of yam and cocoyam tubers. J Appl Biosci 46:3086–3092Google Scholar
  2. Akinoso R, Olatoye KK, Ogunyele OO (2016) Potentials of trifoliate yam (Dioscorea dumetorum) in noodles production. J Food Process Tecnol 7(8):1–6.  https://doi.org/10.4172/2157-7110.1000609 CrossRefGoogle Scholar
  3. AOAC (1984) Official methods of analysis, 14th edn. Association of Official Analytic Chemist, WashingtonGoogle Scholar
  4. Baah FD, Maziya-Dixon B, Asiedu R, Oduro I, Ellis WO (2009) Nutritional and biochemical composition of D. alata (Dioscorea spp.) tubers. J Food Agric Environ 7(2):373–378Google Scholar
  5. Baccou JC, Lambert F, Sauvaire Y (1977) Spectrophotometric method for the determination of total steroidal sapogenin. Analyst 102:458–465CrossRefPubMedGoogle Scholar
  6. Behera KK, Maharana T, Sahoo S, Prusti A (2009) Biochemical quantification of protein, fat, starch, crude fibre, ash and dry matter content indifferent collection of greater yam (Dioscorea alata L.) found in Orissa. Nat Sci 7(7):24–32Google Scholar
  7. Bhandari MR, Kasai T, Kawabata J (2003) Nutritional evaluation of wild yam (Dioscorea spp.) tubers of Nepal. Food Chem 82(4):619–623CrossRefGoogle Scholar
  8. Contreras-Pacheco ML, Santacruz-Ruvalcaba F, Garcia-Fajardo JA, Jesus Sanchez JG, Ruız MAL, Estarron-Espinosa M, Castro-Castro A (2013) Diosgenin quantification, characterisation and chemical composition in a tuber collection of Dioscorea spp. in the state of Jalisco, Mexico. Int J Food Sci Tecnol 48:2111–2118Google Scholar
  9. de Amorim RC (2015) Feature relevance in ward’s hierarchical clustering using the Lp norm. J Classif 32(1):46–62CrossRefGoogle Scholar
  10. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12(1):13–15Google Scholar
  11. Durgawale TP, Durgawale PP, Khanwelkar CC (2016) Quantitative estimation of tannins by HPLC. Der Pharm Lett 8(3):123–126Google Scholar
  12. Eshel D (2011) Non-chemical approaches for postharvest quality management of underground vegetables. Stewart Postharvest Rev 1(3):1–7CrossRefGoogle Scholar
  13. Ezeocha VC, Ojimelukwe PC (2012) The impact of cooking on the proximate composition and anti-nutritional factors of water yam (Dioscorea alata). J Stored Prod Postharvest Res 3(13):172–176Google Scholar
  14. FAO (2010) Fats and fatty acids in human nutrition-Report of an expert consultation. FAO Food and Nutrition, Paper 91, Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  15. Ferede R, Maziya-Dixon B, Alamu OE, Asiedu R (2010) Identification and quantification of major carotenoids of deep yellow-fleshed yam (tropical Dioscorea dumetorum). J Food Agric Environ 8(3&4):160–166Google Scholar
  16. Gawande PA, Nimbhorkar NV, Deshmukh VP, Thakare PV (2015) Assessment of genetic relationships among Dioscorea spp. of Melghat Tiger Reserve Maharashtra of Central India, by using RAPD and its sequences. J Biosci Biotech 4(3):303–312Google Scholar
  17. Hedge JE, Hofreiter BT (1962) Methods in carbohydrate chemistry, 17. In: Whistler RL, BeMiller JN (eds) Academic Press, New York, 420Google Scholar
  18. Hosseinian FS, Li W, Beta T (2008) Measurement of anthocyanins and other phytochemicals in purple wheat. Food Chem 109(4):916–924CrossRefPubMedGoogle Scholar
  19. Jose A, Muhammed R (2015) Extraction and evaluation of anthocyanin from Dioscorea alata (L.) for its application as a natural food colour. Int J Sci Technol 3(9):41–47Google Scholar
  20. Karnjanawipagul P, Nittayanuntawech W, Rojsanga P, Suntornsuk L (2010) Analysis of β-carotene in carrot by spectrophotometry. Mahidol Univ J Pharm Sci 37(1–2):8–16Google Scholar
  21. Lebot V, Malapa R, Molisale T, Marchand JL (2005) Physicochemical characterization of yam (Dioscorea alata L) tubers from Vanuatu. Genet Resour Crop Evol 53(6):1199–1208CrossRefGoogle Scholar
  22. Lenka A, Nedunchezhiyan M (2011) Biochemical composition of two edible aroids. E-Planet 10(2):39–42Google Scholar
  23. Martin FW (1974) Tropical yam and their potential. D. bulbifera. Agric. Learn Book No. 446. USDAGoogle Scholar
  24. Maynard AJ (1970) Methods in food analysis. Academic Press, New York, p 176Google Scholar
  25. Mitra S (2012) Nutritional status of orange-fleshed sweet potatoes in alleviating Vitamin A malnutrition through a food based approach. J Nutr Food Sci 2:160Google Scholar
  26. Mitra S, Tarafdar J (2008) Present status and future prospects of elephant foot yam cultivation in west bengal. In: Palaniswami MS et al. (ed) National seminar on amorphophallus: innovative technologies, July 19–20, 2008, Patna, Bihar-abstract book, status papers and extended summery, pp 25–29Google Scholar
  27. Moorthy SN (1994) Tuber crop starches. Technical Bulletin Series 18. Central Tuber Crops Research Institute, Thiruvananthapuram, Kerala, IndiaGoogle Scholar
  28. Mulualem T, Mekbib F, Hussein S, Gebre NE (2018) Analysis of biochemical composition of yams (Dioscorea spp.) landraces from Southwest Ethiopia. Agrotechnology 7(1):1–8CrossRefGoogle Scholar
  29. Oko AO, Famurewa AC (2015) Estimation of nutritional and starch characteristics of Dioscorea alata (water yam) varieties commonly cultivated in the South-Eastern Nigeria. Br J Appl Sci Technol 6(2):145–152CrossRefGoogle Scholar
  30. Oladeji AE, Bussie MD, Christian OC, Roberts A (2014) Physicochemical and bioactive properties of selected white yam (Dioscorea rotundata) varieties adapted to riverine areas of Nigeria. Afr J Food Sci 8(7):402–409CrossRefGoogle Scholar
  31. Osuntogun A, Adewusi SR, Adewusi A, Ogundiwin JO, Nwasike CC (1989) Effect of cultivar, steeping, and malting on tannin, total polyphenol, and cyanide content of Nigerian sorghum. Cereal Chem 66:87–89Google Scholar
  32. Popoola JO, Adebayo BM, Adegbite AE, Omonhinmin AC, Adewale BD (2017) Fruit morphometric and RAPD evaluation of intraspecific variability in some accessions of African yam bean (Sphenostylis stenocarpa Hochst. ex. A. Rich. Harms). Annu Res Rev Biol 14(4):1–10CrossRefGoogle Scholar
  33. Sanful RE, Engmann FN (2016) Physico-chemical and pasting characteristics of flour and starch from aerial yam. Am J Food Sci Nutr 3(1):1–7Google Scholar
  34. Shajeela PS, Mohan VR, Louis Jesudas L, Tresina-Soris P (2011) Nutritional and antinutritional evaluation of wild yam (Dioscorea spp). Trop Subtrop Agroecosyst 14:723–730Google Scholar
  35. Sharlina MSE, Yaacob WA, Lazim AM, Fazry S, Lim SJ, Abdullah S, Noordin A, Kumaran M (2017) Physicochemical properties of starch from Dioscorea pyrifolia tubers. Food Chem 220:225–232CrossRefGoogle Scholar
  36. Timpa JD, Bruke JJ, Quisenberry JE, Wendt CW (1985) Carbohydrate utilization under stress. Novosibresic-USSR 5:65–69Google Scholar
  37. Topping DL, Clifton DL (2001) SCFA and human colonic function: roles of resistant starch and nonstarch polysaccharides. Physiol Rev 81:1031–1064CrossRefGoogle Scholar
  38. Umetsu Y, Hirata KS, Kudo I (2000) Spectrophotometric determination of saponins in Yucca extract used as food additive. J AOAC Int 836:1451–1454Google Scholar
  39. Umoh EO, Iwe MO (2014) Effects of processing on the nutrient composition of false yam (Icacina trichantha) flour. Off J Niger Inst Food Sci Technol 32(2):1–7Google Scholar
  40. Williams JGK, Kubelik AR, Livak KJ, Antoni Rafalski J, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research 18(22):6531–6535CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Department of Soil Science and Agricultural Chemistry, N M College of AgricultureNavsari Agricultural UniversityNavsariIndia
  2. 2.ASPEE College of HorticultureNavsari Agricultural UniversityNavsariIndia
  3. 3.Department of Genetics and Plant Breeding, N M College of AgricultureNavsari Agricultural UniversityNavsariIndia
  4. 4.Division of Germplasm EvaluationICAR-NBPGRNew DelhiIndia
  5. 5.Faculty of AgricultureUttar Banga Krishi ViswavidyalayaPundibari, Cooch BeharIndia

Personalised recommendations