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EsHO 1 mediated mitigation of NaCl induced oxidative stress and correlation between ROS, antioxidants and HO 1 in seedlings of Eruca sativa: underutilized oil yielding crop of arid region

  • Lovely Mahawar
  • Gyan Singh ShekhawatEmail author
Research Article
  • 22 Downloads

Abstract

Study have focused on NaCl induced HO 1 production and its co-relation to ROS and antioxidant regulation in Eruca sativa. Seedlings were subjected to NaCl stress ranges from 10 to 150 mM. After 96 h of treatment, plants samples were harvested to evaluate the cellular equilibrium and salt tolerance mechanisms through morphological, stress parameters, non enzymatic and antioxidant enzymes. The HO 1 activity was found to be highest at 75 mM NaCl in leaves and roots which were 2.49 and 2.02 folds respectively. The expression of EsHO 1 was also observed and the higher expression was recorded in roots than leaves. The overall activity of other antioxidants (APX and proline) was also found to be higher at 75 mM concentration. The highest HO 1 activity with other antioxidants indicates the decline in LPX and ROS at 75 mM NaCl. The present study concluded that HO 1 helps in amelioration of NaCl stress by working within a group of antioxidants that create the defense machinery in seedlings of E. sativa by manipulating various physiological processes of plants. These findings for the first time suggest the protective role of HO 1 in scavenging ROS in E. sativa under salinity stress.

Keywords

Heme oxygenase 1 Eruca sativa Salinity stress Antioxidants Reactive oxygen species 

Abbreviations

HO 1

Heme oxygenase 1

CAT

Catalase

APX

Ascorbate peroxidase

GPX

Guaiacol peroxidase

SOD

Superoxide dismutase

EtBr

Ethidium bromide

ROS

Reactive oxygen species

TBA

2-Thiobarbituric acid

TCA

Trichloroacetic acid

Notes

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Department of Botany, Centre for Advanced StudiesJai Narain Vyas UniversityJodhpurIndia

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