Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose
- 54 Downloads
Herbaspirillum seropedicae is an endophytic diazotrophic bacterium and a plant growth promoting bacteria. Colletotrichum graminicola causes the anthracnose, one of the most destructive maize diseases worldwide. The main objective of this work was to evaluate the effects of H. seropedicae SmR1 strain on the plant growth and leaf anthracnose of maize plants grown in substrate amended or not amended with humic substances. In the first assay, plants were pre-treated with H. seropedicae and inoculated with C. graminicola at 7, 14 and 21 days after treatment (DAT). In the second assay, plants were treated with H. seropedicae, grown in substrate amended with humic substances and inoculated at 3 and 7 DAT. The anthracnose severity was assessed by measurement of necrotic and chlorotic leaf area, and bacteria were quantified in leaves by quantitative PCR. H. seropedicae did not affect the disease severity in maize leaves, although it efficiently colonized the leaf tissues and it promoted maize leaf growth. Humic substances improved H. seropedicae colonization in maize.
KeywordsColletotrichum graminicola Anthracnose Herbaspirillum seropedicae Plant growth promoting bacteria Diazotrophic bacteria Biocontrol agent
This work was financially supported by the National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN), National Council for Scientific and Technological Development (CNPq), Ministry of Science and Technology, Brazil. The author would like to thank Professor Fábio Lopes Olivares, from University of North Fluminense, Brazil, who kindly provided the humic substances.
This research is part of the doctoral thesis of Pâmela Dall’Asta. Aline C. Velho and Tomás P. Pereira contributed in the Colletotrichum graminicola and Herbaspirillum seropedicae practical procedures, respectively. Professors Ana Carolina M. Arisi and Marciel Stadnik are the supervisors. Pâmela Dall´Asta wrote the basic text and all the authors contributed by reading, revising and improving it, especially the professors Ana Carolina M. Arisi and Marciel Stadnik.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
- Arencibia AD et al (2006) Gluconoacetobacter diazotrophicus Elicitate a Sugarcane Defense Response Against a Pathogenic Bacteria Xanthomonas albilineans Plant Signaling & Behavior 1:265–273Google Scholar
- Baldani VLD, Baldani JI, Dobereiner J (2000) Inoculation of rice plants with the endophytic diazotrophs Herbaspirillum seropedicae and Burkholderia spp. Biol Fertil Soils 30:485–491Google Scholar
- Cabanas CGL, Schiliro E, Valverde-Corredor A, Mercado-Blanco J (2014) The biocontrol endophytic bacterium Pseudomonas fluorescens PICF7 induces systemic defense responses in aerial tissues upon colonization of olive roots. Front Microbiol 5:14. https://doi.org/10.3389/fmicb.2014.00427 Google Scholar
- Couillerot O, Poirier MA, Prigent-Combaret C, Mavingui P, Caballero-Mellado J, Moenne-Loccoz Y (2010b) Assessment of SCAR markers to design real-time PCR primers for rhizosphere quantification of Azospirillum brasilense phytostimulatory inoculants of maize. J Appl Microbiol 109:528–538. https://doi.org/10.1111/j.1365-2672.2010.04673.x Google Scholar
- da Conceicao PM, Vieira HD, Canellas LP, Marques RB, Olivares FL (2008) Corn seed coating with humic acids and endophytic diazotrophic bacteria. Pesquisa Agropecuaria Brasileira 43:545–548Google Scholar
- Elbadry M, Taha RM, Eldougdoug KA, Gamal-Eldin H (2006) Induction of systemic resistance in faba bean (Vicia faba L.) to bean yellow mosaic potyvirus (BYMV) via seed bacterization with plant growth promoting rhizobacteria. J Plant Dis Prot 113:247–251Google Scholar
- ENGL (2015) Definition of minimum performance requirements for analytical methods of GMO testing. European Network of GMO Laboratories. http://gmo-crl.jrc.ec.europa.eu/doc/MPR%20Report%20Application%2020_10_2015.pdf. Accessed Dec 2016
- Gang W, Kloepper JW, Tuzun S (1991) Induction of systemic resistance of cucumber to Colletotrichum-Orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 81:1508–1512Google Scholar
- Guerrero-Molina MF, Lovaisa NC, Salazar SM, Martinez-Zamora MG, Diaz-Ricci JC, Pedraza RO (2015) Physiological, structural and molecular traits activated in strawberry plants after inoculation with the plant growth-promoting bacterium Azospirillum brasilense REC3. Plant Biol 17:766–773. https://doi.org/10.1111/plb.12270 Google Scholar
- Klassen G, Pedrosa FO, Souza EM, Funayama S, Rigo LU (1997) Effect of nitrogen compounds on nitrogenase activity in SMR1. Can J Microbiol 43(9):887–891Google Scholar
- Niu DD, Liu HX, Jiang CH, Wang YP, Wang QY, Jin HL, Guo JH (2011) The plant growth-promoting rhizobacterium Bacillus cereus AR156 induces systemic resistance in Arabidopsis thaliana by simultaneously activating salicylate- and jasmonate/ethylene-dependent signaling pathways. Mol Plant-Microbe Interact 24:533–542. https://doi.org/10.1094/mpmi-09-10-0213 Google Scholar
- Palaversic B, Jukic M, Buhinicek I, Vragolovic A, Kozic Z (2009) Breeding maize for resistance to stalk anthracnose. Maydica 54:229–232Google Scholar
- Ribaudo CM, Rondanini DP, Trinchero GD, Cura JA (2006) Effect of Herbaspirillum seropedicae inoculation on maize nitrogen metabolism. Maydica 51:481–485Google Scholar
- Stets MI, Alqueres S, Souza EM, Pedrosa FO, Schmid M, Hartmann A, Cruz LM (2015) Quantification of Azospirillum brasilense FP2 in wheat roots by strain-specific qPCR. Appl Environ Microbiol. https://doi.org/10.1128/aem.01351-15
- Weihmann F, Eisermann I, Becher R, Krijger JJ, Hubner K, Deising HB, Wirsel SGR (2016) Correspondence between symptom development of Colletotrichum graminicola and fungal biomass, quantified by a newly developed qPCR assay, depends on the maize variety. BMC Microbiol 16:14. https://doi.org/10.1186/s12866-016-0709-4 Google Scholar