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Physiology and Molecular Biology of Plants

, Volume 25, Issue 1, pp 167–176 | Cite as

Herbaspirillum seropedicae promotes maize growth but fails to control the maize leaf anthracnose

  • Pâmela Dall’Asta
  • Aline Cristina Velho
  • Tomás Pellizzaro Pereira
  • Marciel João Stadnik
  • Ana Carolina Maisonnave ArisiEmail author
Research Article
  • 54 Downloads

Abstract

Herbaspirillum seropedicae is an endophytic diazotrophic bacterium and a plant growth promoting bacteria. Colletotrichum graminicola causes the anthracnose, one of the most destructive maize diseases worldwide. The main objective of this work was to evaluate the effects of H. seropedicae SmR1 strain on the plant growth and leaf anthracnose of maize plants grown in substrate amended or not amended with humic substances. In the first assay, plants were pre-treated with H. seropedicae and inoculated with C. graminicola at 7, 14 and 21 days after treatment (DAT). In the second assay, plants were treated with H. seropedicae, grown in substrate amended with humic substances and inoculated at 3 and 7 DAT. The anthracnose severity was assessed by measurement of necrotic and chlorotic leaf area, and bacteria were quantified in leaves by quantitative PCR. H. seropedicae did not affect the disease severity in maize leaves, although it efficiently colonized the leaf tissues and it promoted maize leaf growth. Humic substances improved H. seropedicae colonization in maize.

Keywords

Colletotrichum graminicola Anthracnose Herbaspirillum seropedicae Plant growth promoting bacteria Diazotrophic bacteria Biocontrol agent 

Notes

Acknowledgements

This work was financially supported by the National Institute of Science and Technology-Biological Nitrogen Fixation (INCT-FBN), National Council for Scientific and Technological Development (CNPq), Ministry of Science and Technology, Brazil. The author would like to thank Professor Fábio Lopes Olivares, from University of North Fluminense, Brazil, who kindly provided the humic substances.

Author contributions

This research is part of the doctoral thesis of Pâmela Dall’Asta. Aline C. Velho and Tomás P. Pereira contributed in the Colletotrichum graminicola and Herbaspirillum seropedicae practical procedures, respectively. Professors Ana Carolina M. Arisi and Marciel Stadnik are the supervisors. Pâmela Dall´Asta wrote the basic text and all the authors contributed by reading, revising and improving it, especially the professors Ana Carolina M. Arisi and Marciel Stadnik.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  1. 1.Laboratory of Molecular Biology, Department of Food Science and TechnologyFederal University of Santa CatarinaFlorianópolisBrazil
  2. 2.Laboratory of Plant Pathology, Department of PhytotechnyFederal University of Santa CatarinaFlorianópolisBrazil

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