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Physiology and Molecular Biology of Plants

, Volume 25, Issue 1, pp 145–157 | Cite as

Antioxidant potential of barley genotypes inoculated with five different pathotypes of Puccinia striiformis f. sp. hordei

  • Prabhjot Singla
  • Rachana D. BhardwajEmail author
  • Simarjit Kaur
  • Jaspal Kaur
Research Article
  • 46 Downloads

Abstract

The stripe rust caused by the fungal pathogen, Puccinia striiformis f. sp. hordei in barley (Hordeum vulgare L.) is a global problem that threatens the production of barley. The present study examined the disease reaction, free radical scavenging potential, non-enzymatic antioxidants like total phenols, o-dihydroxy phenols, flavonoids along with total chlorophyll, chlorophyll a, chlorophyll b and total carotenoids of the four barley genotypes viz. Jyoti (susceptible), RD2900, RD2901 and RD2552 (resistant) infected with five different pathotypes (M, G, 57, Q and 24) of P. striiformis f. sp. hordei. The disease reaction showing RD2901 in the category of immune to very resistant genotype followed by RD2552 in immune to resistant and RD2900 as moderately resistant and Jyoti as susceptible, which was well correlated with biochemical studies. RD2901 possessed higher antioxidant potential in terms of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH·) scavenging activity, ferric reducing antioxidant power, reducing power and nitric oxide scavenging activity under control conditions and were maintained sufficiently high on inoculation with different pathotypes (M, G, 57, Q and 24) of P. striiformis f. sp. hordei. Further, these free radical scavenging activities showed the positive correlation with total phenols, o-dihydroxy phenols, flavonoids which in turn might be contributing in tolerance behaviour of this genotype. However, Jyoti with sensitive behaviour towards M, G, and 24 pathotypes depicted minimum DPPH activity and reducing power under control conditions.

Keywords

Hordeum vulgarePuccinia striiformis f. sp. hordei Free radicals Antioxidants 

Abbreviations

ROS

Reactive oxygen species

1O2

Singlet oxygen

H2O2

Hydrogen peroxide

O2·−

Superoxide anion radical

OH·

Hydroxyl radical

NO

Nitric oxide

DPPH

2,2-diphenyl-1-picrylhydrazyl

FRAP

Ferric reducing antioxidant power

TPTZ

2,4,6-Tri-(2-pyridyl)5-triazine

TCA

Trichloroacetic acid

ANOVA

Analysis of variance

Notes

Acknowledgements

The authors are thankful to Department of Plant Breeding and Genetics, Punjab Agricultural University for providing barley genotypes and helping in field work.

Compliance with ethical standards

Conflict of interest

Authors declare that they have no conflicts of interest.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  • Prabhjot Singla
    • 1
  • Rachana D. Bhardwaj
    • 1
    Email author
  • Simarjit Kaur
    • 2
  • Jaspal Kaur
    • 2
  1. 1.Department of BiochemistryPunjab Agricultural UniversityLudhianaIndia
  2. 2.Department of Plant Breeding and GeneticsPunjab Agricultural UniversityLudhianaIndia

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