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Physiology and Molecular Biology of Plants

, Volume 25, Issue 1, pp 13–29 | Cite as

Gene expression analysis of bud burst process in European hazelnut (Corylus avellana L.) using RNA-Seq

  • Musa KavasEmail author
  • Aslıhan Kurt Kızıldoğan
  • Hüseyin İrfan Balık
Research Article
  • 133 Downloads

Abstract

The control of bud burst process depending on temperature is crucial factor in woody perennial plants to survive in unfavorable ecological conditions. Although it has important economic and agronomic values, little information is available on the molecular mechanism of the bud burst process in Corylus avellana. Here for the first time, we conducted a de novo transcriptome-based experiment using eco-dormant leaf bud tissues. Four transcriptome libraries were constructed from the leaf bud tissues and sequenced via Illumina platform. Transcriptome analysis revealed 86,394 unigenes with a mean length of 1189 nt and an N50 of 1916 nt. Among these unigenes, 63,854 (73.78%) of them were annotated by at least one database. De novo assembled transcripts were enriched in phenylpropanoid metabolism, phytohormone biosynthesis and signal transduction pathways. Analyses of phytohormone-associated genes revealed important changes during bud burst, in response to gibberellic acid, auxin, and brassinosteroids. Approximately 2163 putative transcription factors were predicted, of which the largest number of unique transcripts belonged to the MYB transcription factor family. These results contribute to a better understanding of the regulation of bud burst genes in perennial plants.

Keywords

Bud burst Dormancy Hazelnut Phenylpropanoid metabolism Phytohormone biosynthesis RNA-Seq 

Notes

Acknowledgements

This research was supported by a Research Fund of The Scientific and Technological Research Council of Turkey (114O800).

Compliance with ethical standards

Data archiving statement

The sequencing datasets were deposited in NCBI SRA database under project number accession PRJNA316492. The experiment accession numbers of cDNA libraries obtained from the Palaz 1, Palaz 2, Çakıldak 1 and Çakıldak are SRX1665375, SRX1665376, SRX1665377, and SRX1665378, respectively. Assembled transcript sequences was deposited in TSA (SUB4195016).

Conflict of interest

The authors have no conflict of interest.

Supplementary material

12298_2018_588_MOESM1_ESM.docx (1.3 mb)
Supplementary material 1 (DOCX 1348 kb)

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2018

Authors and Affiliations

  1. 1.Department of Agricultural Biotechnology, Faculty of AgricultureOndokuz Mayıs UniversitySamsunTurkey
  2. 2.Giresun Hazelnut Research StationMinistry of Food, Agriculture and LivestockGiresunTurkey

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