Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT
- 12 Downloads
Two gene targeted markers i.e. CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism were applied to analyze the genetic stability of in vitro propagated plants of Bauhinia racemosa Lam. multiplied by enhanced axillary shoot proliferation of mature tree derived nodal explant. Nine randomly selected micropropagated plants of 1 year age were subjected to molecular analysis. The isolated genomic DNA samples were subjected to PCR amplification with a total of 61 primers (25 CBDP and 36 SCoT) out of which 39 primers (21 CBDP and 18 SCoT) produced scorable amplicons. A total of 97 and 88 clear, distinct and reproducible amplicons were produced by CBDP and SCoT primers, respectively. The monomorphic banding pattern obtained through all the tested primers corroborated the true to type nature of in vitro propagated plants of B. racemosa.
KeywordsGene targeted Genetic stability Molecular markers Plant regeneration Somaclonal variation
Udit Sharma gratefully acknowledges University Grants Commission, New Delhi for providing financial assistance [F.17-78/2008 (SA-I)] in the form of Junior and Senior research fellowship.
Compliance with ethical standards
Conflict of interest
Authors declare that they have no conflict of interest.
- Chavan JJ, Gaikwad NB, Kshirsagar PR, Umdale SD, Bhat KV, Dixit GB, Yadav SR (2015) Highly efficient in vitro proliferation and genetic stability analysis of micropropagated Ceropegia evansii by RAPD and ISSR markers: a critically endangered plant of Western Ghats. Plant Biosyst 149:442–450CrossRefGoogle Scholar
- Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15Google Scholar
- Panda P, Das D, Dash P, Ghosh G (2015) Therapeutic potential of Bauhinia racemosa—a mini review. Int J Pharm Sci Rev Res 32:169–179Google Scholar
- Prasad P, Nautiyal AR (1996) Physiology of germination in Bauhinia: involvement of seed coat in inhibition of germination in B. racemosa Lam. seeds. Seed Sci Technol 24:305–308Google Scholar
- Ravindranath NH, Bhat DM, Swamy VS (2003) Nursery manual for forest tree species. University Press, HyderabadGoogle Scholar
- Sathish D, Vasudevan V, Theboral J, Elayaraja D, Appunu C, Siva R, Manickavasagam M (2018) Efficient direct plant regeneration from immature leaf roll explants of sugarcane (Saccharum officinarum L.) using polyamines and assessment of genetic fidelity by SCoT markers. Vitro Cell Dev Biol Plant 54:399–412CrossRefGoogle Scholar
- Singh V, Pandey RP (1998) Ethnobotany of Rajasthan, India. Scientific Publisher, JodhpurGoogle Scholar
- Tiwari G, Singh R, Singh N, Choudhary DR, Paliwal R, Kumar A, Gupta V (2016) Study of arbitrarily amplified (RAPD and ISSR) and gene targeted (SCoT and CBDP) markers for genetic diversity and population structure in Kalmegh [Andrographis paniculata (Burm. f.) Nees]. Ind Crops Prod 86:1–11CrossRefGoogle Scholar