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Evaluation and clustering on salt-tolerant ability in rice genotypes (Oryza sativa L. subsp. indica) using multivariate physiological indices

  • Wasinee Pongprayoon
  • Rujira Tisarum
  • Cattarin Theerawittaya
  • Suriyan Cha-um
Research Article
  • 45 Downloads

Abstract

Salinity is a major abiotic stress that affects plant growth and development, especially in rice crop as it is a salt susceptible crop. Therefore, a wide range of rice genetic resources are screened in the germplasm banks to identify salt tolerant cultivars. The objective of this investigation was to develop effective indices for the classification of salt tolerant rice genotypes among Pathumthani 1, Khao Dawk Mali 105 (KDML 105), RD31, RD41, Suphanburi 1, RD43, RD49 and Riceberry. Rice seedlings were hydroponically grown with 10 dS m−1 NaCl treatment or without NaCl treatment (to serve as control) (WP; 2 dS m−1). Standard evaluation system peaked at a score of 9 in Pathumthani 1 and KDML 105, after 21 days of salt treatment, leading to leaf chlorosis, leaf burns and plant death. Chlorophyll a, chlorophyll b and total carotenoids were maintained better in the salt-stressed leaves of rice cvs. Riceberry and RD43, as compared to other cultivars. Salt stress induced a remarkable increase in the free proline accumulation (by 8.38 folds) in cv. Riceberry. Overall growth performance in rice cv. Riceberry was retained, whereas it declined in other cultivars. After 21 days of NaCl treatment at a concentration of 10 dS m−1, eight rice cultivars were classified into 3 groups based on multivariate physio-morphological indices, Group I: salt-tolerant rice, including cv. Riceberry; Group II: moderately salt tolerant, consisting of RD31, RD41, Suphanburi 1, RD43 and RD49 cultivars; Group III: salt-sensitive cultivars, namely Pathumthani 1 and KDML 105.

Keywords

Multivariate cluster ranking Photosynthetic pigments Free proline Indica rice Salt tolerance 

Notes

Acknowledgements

The authors are grateful to Suphan Buri Rice Research Center (Rice Research Institute, Department of Agriculture, Ministry of Agriculture and Cooperative, Thailand) for providing rice seeds. We would like to thank Faculty of Science, Burapha University and National Center for Genetic Engineering and Biotechnology (BIOTEC) for funding support and lab facilities.

Authors contribution

WP conducted the research project, report to funding agency, analyzed the data and wrote a first draft of manuscript, CT performed the experiment layout, free proline assay and analysis, RT analyzed the cluster ranking, critical revision of the data and performed the experiment, and SC performed the secondary data analysis and finished the final version of manuscript.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Prof. H.S. Srivastava Foundation for Science and Society 2019

Authors and Affiliations

  1. 1.Department of Biology, Faculty of ScienceBurapha UniversityChon BuriThailand
  2. 2.National Center for Genetic Engineering and Biotechnology (BIOTEC)National Science and Technology Development Agency (NSTDA)Pathum ThaniThailand

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