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Cinnamon Attenuated Long-Term IGT-Induced Retinal Abnormalities via Regulation of Glucose Homeostasis in Neonatal Streptozotocin Induced Rat Model

  • Sivakesava Rao Kommula
  • Sai Santhosh Vadakattu
  • Srinivas Myadara
  • Uday Kumar Putcha
  • Raghava Rao Tamanam
  • Suryanarayana PallaEmail author
Original Research Article
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Abstract

Diabetic retinopathy (DR) is one of the major causes of blindness all over the world. According to the previous studies, impaired glucose tolerance (IGT) has been linked to retinal dysfunction/vascular damage. Decreased retinal function is an initial event of early DR. Although the biochemical and molecular events are not fully understood, glial activation, angiogenesis and oxidative stress are some of the pathways associated with early retinal abnormalities. Since IGT is associated with development of retinal dysfunction/vascular damage; as a preventive strategy, we have studied beneficial effect of Cinnamon as a hypoglycaemic agent on long-term IGT induced retinal abnormalities using neonatal streptozotocin (nSTZ) rat model. Control, IGT rats were maintained on AIN-93M diet alone and another set of IGT rats were maintained on AIN-93M diet with 3% Cinnamon for 8 months. At the end of the study, untreated IGT rats developed retinal functional abnormalities as assessed by electroretinogram (ERG) and the retinal structure did not alter as assessed by H&E staining. Further, increase in expressions of GFAP, VEGF and decreased expression of rhodopsin in untreated IGT rat retinas. 4-HNE, a marker of oxidative stress was also elevated in IGT state. Supplementation of Cinnamon to IGT rats had lowered fasting and postprandial glucose levels and also prevented retinal functional abnormalities. Further, Cinnamon protected photoreceptor cell damage, suppressed glial activation, angiogenesis and oxidative stress as there was an improved rhodopsin expression, inhibited elevated expressions of GFAP, VEGF and 4-HNE respectively. In conclusion, Cinnamon attenuated IGT induced retinal abnormalities probably through its hypoglycemic property.

Keywords

Cinnamon Impaired glucose tolerance Retinal abnormalities Neonatal streptozotocin 

Notes

Funding

This work was supported by the Department of Biotechnology, Government of India (Grant No: BT/PR3446/BRB/10/969/2011) and National Institute of Nutrition (Indian Council of Medical research, Government of India) through intramural funding (#12-BS11).

Compliance with Ethical Standards

Conflict of interest

All authors declare no conflict of interest.

Ethical Approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Copyright information

© Association of Clinical Biochemists of India 2019

Authors and Affiliations

  • Sivakesava Rao Kommula
    • 1
  • Sai Santhosh Vadakattu
    • 1
  • Srinivas Myadara
    • 2
  • Uday Kumar Putcha
    • 2
  • Raghava Rao Tamanam
    • 3
  • Suryanarayana Palla
    • 1
    Email author
  1. 1.Lipid Chemistry DivisionNational Institute of NutritionJamai-Osmania, HyderabadIndia
  2. 2.Pathology DivisionNational Institute of NutritionHyderabadIndia
  3. 3.Biochemistry DivisionAndhra UniversityVisakhapatnamIndia

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