MicroRNAs (miRNAs) are small, endogenous, non-coding, single stranded RNAs which play a role in the regulation of gene expression and function. Therefore, the analysis of differentially expressed miRNAs are of great importance in disease diagnosis. This study is focussed on the differential expression of miRNAs in serum of PCOS subjects compared to control and their correlation with metabolic and endocrine parameters. Anthropometry, hormone concentrations and biochemical characteristics were measured in healthy (n = 20) and PCOS (n = 20) subjects. MiR-24, miR-29a and miR-502-3p were determined in serum by quantitative RT-PCR. The levels of miR-24 was significantly decreased in PCOS subjects (P = 0.00) compared to control. No significant difference was observed in the levels of miR-29a and miR-502-3p in PCOS and control subjects. MiR-24 showed significant inverse correlation with BMI, glucose, insulin, FIRI, HOMA, LH, testosterone, TG, and LH:FSH ratio whereas HDL levels showed significant positive association with miR-24 and miR-29a. LH showed significant negative association with miR-29a. No correlation was observed between the expression of miR-502-3p with any of the studied parameters. The receiver operating characteristic curve for miR-24 alone showed a significant discriminative capacity. The study suggests that serum miR-24 analysis in PCOS patients could be of diagnostic value that can be used as a biomarker for PCOS.
Polycystic ovary syndrome Body mass index Luteinizing hormone Follicle stimulating hormone miRNA-24
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The authors thank Dr. K. Lavanya kumari, M.B.B.S., M.D, (RMMC & H) and Dr. A. Brinda, M.D., D.G.O, Gynaecologist for their help in providing the blood samples of control and PCOS subjects and also all the participants in the study. We acknowledge DST-FIST and UGC-SAP support for the facilities developed in the Department of Biochemistry and Biotechnology, Annamalai University for executing the present study.
This study was supported by University Grants Commission (UGC) in form of Research Fellowship in Sciences for Meritorious Students (RFSMS) to Ms. Dipti Nanda.
Compliance with Ethical Standards
Conflict of interest
The authors disclose that they do not have any conflict of interest.
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