Indian Journal of Clinical Biochemistry

, Volume 33, Issue 2, pp 171–177 | Cite as

Protective Effect of Allyl Isothiocyanate on Glycoprotein Components in 7,12-dimethylbenz(a)anthracene Induced Mammary Carcinoma in Rats

  • Thangarasu Rajakumar
  • Pachaiappan Pugalendhi
  • Subbaiyan Thilagavathi
Original Article

Abstract

The present study aimed to investigate the protective effect of allyl isothiocyanate (AITC) on glycoprotein components in 7,12-dimethylbenz(a)anthracene (DMBA) induced mammary carcinogenesis in female Sprague–Dawley rats. Mammary tumor was induced by a single dose of DMBA (25 mg/rat) injected subcutaneously near mammary gland. The levels of glycoprotein components such as hexose, hexosamine and sialic acid were analyzed colorimetrically in plasma, mammary and liver tissues. We observed an increased levels of glycoprotein components in plasma, mammary and liver tissues in cancer bearing rats. It was further confirmed by Periodic Acid Schiff staining in mammary and liver tissues. Upon oral administration of AITC to DMBA injected rats, the abnormal changes were reverted back to near normal levels and biochemical findings are supported by histological analysis. This could be due to the anti-neoplastic potential of AITC against DMBA-induced mammary carcinogenesis. The result shows that AITC has the potential to inhibit abnormal glycosylation that favors neoplastic transformation.

Keywords

Allyl isothiocyanate 7,12-dimethylbenz(a)anthracene Glycoprotein components Mammary carcinogenesis 

Notes

Acknowledgements

We gratefully acknowledged the financial assistance from Indian Council of Medical Research (ICMR), in the form of Senior Research Fellowship (SRF), New Delhi, India to the first author Mr. T. Rajakumar.

Funding

This study was funded by Indian Council of Medical Research (ICMR), Government of India (Grant No. 3/2/2/189/2013/NCD-III (OPA-27129)).

Compliance with Ethical Standards

Conflict of interest

The first author (Mr. T. Rajakumar) has received Senior Research Fellowship (SRF), from Indian Council of Medical Research (ICMR), Government of India. The corresponding author (Dr. P. Pugalendhi) and last author (Mrs. S. Thilagavathi) declares that there are no conflicts of interest.

Ethical Approval

This study was approved by the Institutional Animal Ethics Committee (IAEC), regulated by the Committee for the Purpose of Control and Supervision of Experimental Animals (CPCSEA) (Reg. No. 160/1999/CPCSEA and Proposal No. 983).

Informed Consent

This article does not contain any studies with human participants.

References

  1. 1.
    Gönenç A, Erten D, Aslan S, Akinci M, Simşek B, Torun M. Lipid peroxidation and antioxidant status in blood and tissue of malignant breast tumor and benign breast disease. Cell Biol Int. 2006;30:376–80.CrossRefPubMedGoogle Scholar
  2. 2.
    Michor F, Iwasa Y, Nowak MA. Dynamics of cancer progression. Nat Rev Cancer. 2004;4:197–205.CrossRefPubMedGoogle Scholar
  3. 3.
    Koumoutsakos P, Pivkin I, Milde F. The fluid mechanics of cancer and its therapy. Annu Rev Fluid Mech. 2013;45:325–55.CrossRefGoogle Scholar
  4. 4.
    Sell S. Cancer-associated carbohydrates identified by monoclonal antibodies. Hum Pathol. 1990;21:1003–19.CrossRefPubMedGoogle Scholar
  5. 5.
    Murray RK. Glycoproteins. Harper’s biochemistry. 24th ed. Appleton and Lange. USA 1996.Google Scholar
  6. 6.
    Tarner GA, Skillen AW, Buamah P, Guthrie D, Welsh J, Harrison J, et al. Relation between raised concentrations of fucose, sialic acid, and acute phase proteins in serum from patients with cancer: choosing suitable serum glycoproteins markers. J Clin Pathol. 1985;38:588–92.CrossRefGoogle Scholar
  7. 7.
    Warren L, Buck CA, Tuszynski GP. Glycopeptide changes and malignant transformation. A possible role for carbohydrate in malignant behavior. Biochim Biophys Acta. 1978;516:97–127.PubMedGoogle Scholar
  8. 8.
    Dube DH, Bertozzi CR. Glycans in cancer and inflammation-potential for therapeutics and diagnostics. Nat Rev Drug Discov. 2005;4:477–88.CrossRefPubMedGoogle Scholar
  9. 9.
    Patel PS, Adhvaryu SG, Balar DB, Parikh BJ, Shah PM. Clinical application of serum levels of sialic acid, fucose and seromucoid fraction as tumour markers in human leukemias. Anticancer Res. 1994;14:747–51.PubMedGoogle Scholar
  10. 10.
    Hwang ES, Lee HJ. Allyl isothiocyanate and its N-acetylcysteine conjugate suppress metastasis via inhibition of invasion, migration, and matrix metalloproteinase-2/-9 activities in SK-Hep 1 human hepatoma cells. Exp Biol Med. 2006;231:421–30.CrossRefGoogle Scholar
  11. 11.
    Manesh C, Kuttan G. Anti-tumour and anti-oxidant activity of naturally occurring isothiocyanates. J Exp Clin Cancer Res. 2003;22:193–9.PubMedGoogle Scholar
  12. 12.
    Rajakumar T, Pugalendhi P, Thilagavathi S. Dose response chemopreventive potential of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats. Chem Biol Interact. 2015;231:35–43.CrossRefPubMedGoogle Scholar
  13. 13.
    Chidambaram N, Baradarajan A. Influence of selenium on glutathione and some associated enzymes in rats with mammary tumor induced by 7,12-dimethylbenz(a)anthracene. Mol Cell Biochem. 1996;156:101–7.CrossRefPubMedGoogle Scholar
  14. 14.
    Elanchezhiyan C, Kumaravel K, Bhat BA, Sethupathy S. Protective role of Helicteres isora plant extract on plasma and tissue glycoprotein components in streptozotocin induced hyperglycemic rats. Phcog J. 2014;6:86–91.CrossRefGoogle Scholar
  15. 15.
    Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem. 1957;226:497–509.PubMedGoogle Scholar
  16. 16.
    Niebes P. Determination of enzymes and degradation products of glycosaminoglycans metabolism in the serum of healthy and varicose subjects. Clin Chim Acta. 1972;42:399–408.CrossRefGoogle Scholar
  17. 17.
    Elson LA, Morgan WT. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J. 1933;27:1824–8.CrossRefPubMedPubMedCentralGoogle Scholar
  18. 18.
    Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem. 1959;234:1971–5.PubMedGoogle Scholar
  19. 19.
    Yamabayashi S. Periodic acid-Schiff-alcian blue: a method for the differential staining of glycoproteins. Histochem J. 1987;19:565–71.CrossRefPubMedGoogle Scholar
  20. 20.
    Dennis JW, Granovsky M, Warren CE. Glycoprotein glycosylation and cancer progression. Biochim Biophys Acta. 1999;1473:21–34.CrossRefPubMedGoogle Scholar
  21. 21.
    Gemayel R, Fortpied J, Rzem R, Vertommen D, Veiga-da-Cunha M, Van Schaftingen E. Many fructosamine 3-kinase homologues in bacteria are ribulosamine/erythrulosamine 3-kinases potentially involved in protein deglycation. FEBS J. 2007;274:4360–74.CrossRefPubMedGoogle Scholar
  22. 22.
    Dharmendra V, Palanivelu S, Panchanadham S. Immunomodulatory effect of Kalpaamruthaa on 7,12 dimethyl benz(a)anthracene induced mammary carcinoma studied in rats. Com Clin Pathol. 2014;23:1087–94.CrossRefGoogle Scholar
  23. 23.
    Ramprasath VR, Akila G, Shanthi P, Sachdanandam P. Biochemical evaluation of glycoprotein components, Lysosomal enzymes and marker enzymes upon kalpaamruthaa administration in experimental mammary carcinoma rats. J Health Sci. 2007;53:644–54.CrossRefGoogle Scholar
  24. 24.
    Purushothaman A, Nandhakumar E, Sachdanandam P. Anticancer effect of shemamruthaa (a phytochemical formulation) on 7, 12-Dimethylbenz(a)anthracene induced mammary carcinoma in rats. Asian J Pharm Clin Res. 2012;5:101–7.Google Scholar
  25. 25.
    Veena K, Shanthi P, Sachdanandam P. Protective effect of Kalpaamruthaa on altered glycoproteins component levels and membrane stability in mammary carcinoma. Int J Cancer Res. 2006;2:315–29.CrossRefGoogle Scholar
  26. 26.
    Jagadeesan AJ, Langeswaran K. Gowtham kumar S, Revathy R, Balasubramanian MP. Chemopreventive potential of diosgenin on modulating glycoproteins, TCA cycle enzymes, carbohydrate metabolising enzymes and biotransformation enzymes against N-methyl-N-nitrosourea induced mammary carcinogenesis. Int. J Pharm Pharm Sci. 2013;5:575–82.Google Scholar
  27. 27.
    Nandakumar N, Jayaprakash R, Balasubramanian MP. Influence of hesperidin on renal cell surface glycoprotein content, nucleic acids, lysosomal enzymes and macromolecules against 7, 12-dimethylbenz [a] anthracene induced experimental breast carcinoma. J Exp Ther Oncol. 2012;9:265–80.PubMedGoogle Scholar
  28. 28.
    Singhal A, Hakomori S. Molecular changes in carbohydrate antigens associated with cancer. Bioassays. 1990;12:223–30.CrossRefGoogle Scholar
  29. 29.
    Buijs JT, Cleton AM, Smit VT, Löwik CW. E Papapoulos S, Pluijm GV. Prognostic significance of periodic acid-Schiff-positive patterns in primary breast cancer and its lymph node metastases. Breast Cancer Res Treat. 2004;84:117–30.CrossRefPubMedGoogle Scholar
  30. 30.
    Arivazhagan L, Sorimuthu Pillai S. Tangeretin, a citrus pentamethoxyflavone, exerts cytostatic effect via p53/p21 up-regulation and suppresses metastasis in 7,12-dimethylbenz(α)anthracene-induced rat mammary carcinoma. J Nutr Biochem. 2014;25:1140–53.CrossRefPubMedGoogle Scholar
  31. 31.
    Gowtham Kumar S, Ramakrishnan V, Madhusudhanan N, Balasubramanian MP. Antioxidant activity of Allyl isothiocyanate [AITC] against N-nitrosodiethylamine induced experimental liver carcinogenesis. J Pharm Res. 2011;4:3690–4.Google Scholar

Copyright information

© Association of Clinical Biochemists of India 2017

Authors and Affiliations

  • Thangarasu Rajakumar
    • 1
  • Pachaiappan Pugalendhi
    • 1
  • Subbaiyan Thilagavathi
    • 1
  1. 1.Department of Biochemistry and Biotechnology, Faculty of ScienceAnnamalai UniversityChidambaramIndia

Personalised recommendations