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Efficacy of Dichlorophenolindophenol (DCIP) as Screening Test for Hb E: Revisited

  • Prakas Kumar Mandal
  • K. S. Nataraj
  • Shuvra Neel BaulEmail author
  • Malay Kumar Ghosh
  • Tuphan Kanti Dolai
Original Article
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Abstract

Hb E-beta thalassemia is a major public health problem in West Bengal, India and is the predominant symptom producing thalassemia in this part of the country. To search for an easy, reliable and cost effective screening method for HbE that can be used at the community level where more sophisticated methods are not readily available. And the DCIP test was performed for the purpose. Blood samples of 425 asymptomatic family members from 80 diagnosed cases of HbE beta Thalassemia patients were tested for Hb, RBC indices, DCIP test, HPLC, and in discordant cases confirmed by DNA mutation analysis. The present study shows DCIP screening test to have a sensitivity, specificity, positive predictive value and negative predictive value of 96.39%, 97.43%, 96.39% and 97.43% respectively. It also shows a false positive rate and false negative rate in 2.56% and 4.6% cases respectively. The advantage with DCIP over HPLC is that it can be easily performed at the community level by a person with minimum technical skill, few samples (even a single sample) can be tested at time, at a low cost.

Keywords

Hemoglobin E Screening test HPLC DCIP 

Notes

Funding

None.

Compliance with Ethical Standards

Conflict of interest

The authors declare no potential conflicts of interest.

Ethical Approval

The study was approved by the Institutional Ethical Committee (IEC).

Patient Consent

Written consent taken from each patient and/or their legal guardians at the entry into the study.

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Copyright information

© Indian Society of Hematology and Blood Transfusion 2019

Authors and Affiliations

  1. 1.Department of HematologyCSTM and NRS Medical CollegeKolkataIndia
  2. 2.KolkataIndia
  3. 3.Mazumdar Shaw Cancer CenterNarayana HealthBengaluruIndia
  4. 4.Department of HematologyNRS Medical CollegeKolkataIndia

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