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Parvovirus B19 Infection in Human Bone Marrow Mesenchymal Stem Cells Affects Gene Expression of IL-6 and TNF-α and also Affects Hematopoietic Stem Cells Differentiation

  • Mahin Behzadi Fard
  • Saeid KavianiEmail author
  • Amir Atashi
Original Article
  • 30 Downloads

Abstract

Parvovirus B19 (B19V) has been known to induce transient erythroid aplasia, cytopenia and aplastic anemia. This virus persists in bone marrow mesenchymal stem cells (HBMSCs) of some immunocompetent individuals several years after primary infection. In B19V infected erythroid progenitor cells, the virus induces transactivation of Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) gene expression. Due the critical role of HBMSCs in bone marrow niche and inhibitory effect of inflammatory cytokines on hematopoiesis, the aim of this study was to investigate the effect of B19V on IL-6 and TNF-α gene expression intransfected cells. In addition we assessed the clonogenicity potential of cord blood CD34+ stem cells that were co-cultured with infected cells. After 24 h of transfection, quantitative mRNA expression of IL-6 and TNF-α was evaluated and human cord blood CD34+ HSC were cultured with the transfected cells. At the end of 7 days of culture, HSCs colony forming units (CFUs) assay was performed. Our findings demonstrated statistically significant (18.1 and 21.9 fold) increase of TNF-α and IL-6 gene expression respectively and decrease in burst forming unit-erythrocyte (BFU-E) and colony forming unit-erythrocyte (CFU-E) enumeration(p < 0.05). We concluded that, inducing inflammatory cytokines gene expression in B19V-infected HBMSCs might influence on bone marrow microenvironment and hematopoiesis.

Keywords

Colony forming assay Hematopoietic stem cells Human bone marrow mesenchymal stem cells Inflammatory cytokines Parvovirus B19 

Abbreviations

B19V

Parvovirus B19

HBMSCs

Human bone marrow mesenchymal stem cells

IL-6

Interleukin-6

TNF-α

Tumor necrosis factor-α

NS1

Nonstructural protein 1

VP1/VP2

Viral proteins1/2

HHV-6

Human herpes virus 6

VZV

Varicella-zoster virus

EBV

Epstein–Barr virus

CMV

Cytomegalovirus

HAV/HCV

Hepatitis A/C viruses

HIV

Human immunodeficiency virus

FBS

Fetal bovine serum

DMEM

Dulbecco’s modified eagle’s medium

CFU-GEMM

Colony forming unit-granulocyte/erythrocyte monocyte/megakaryocyte

CFU-GM

Colony forming unit-granulocyte/monocyte

CFU-G

Colony forming unit-granulocyte

CFU-M

Colony forming unit-monocyte

BFU-E

Burst forming unit-erythrocyte

CFU-E

Colony forming unit-erythrocyte

EPCs

Erythroid progenitor cells

Notes

Acknowledgements

The authors are thankful to Dr. Giorgio Gallinella, Department of Pharmacy and Biotechnology, S. Orsola-Malpighi Hospital - Microbiology, University of Bologna, Bologna, Italy for kind gifting pHI0-plasmid. The results described in this paper were part of a student thesis and was financially supported by Deputy of Research Affairs and Faculty of Medical Sciences at Tarbiat Modares University, Tehran, Iran.

Authors’ Contribution

AA and SK designed the experiments. MBF carried out the experiments, analyzed the data and wrote the manuscript.

Funding

This work was supported by a grant from Tarbiat Modares University of Medical Sciences (Grant Number IR.TUM.REC0.53934).

Compliance with Ethical Standards

Conflict of interest

The authors report no conflicts of interest in this work.

Ethical Approval

This study was approved by the local ethics committee of Tarbiat Modares University of Medical Sciences (Approval Number IR.TMU.REC0.1394.288).

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Copyright information

© Indian Society of Hematology and Blood Transfusion 2019

Authors and Affiliations

  • Mahin Behzadi Fard
    • 1
  • Saeid Kaviani
    • 2
    Email author
  • Amir Atashi
    • 3
  1. 1.Department of Hematology and Blood Banking, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Hematology, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  3. 3.Stem Cell and Tissue Engineering Research Center, Shahroud University of Medical SciencesShahroudIran

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