Vitamin D Deficiency and Its Association with Inflammatory Markers, Lipid Profile and Regulatory T-cells in Pediatric Sickle Cell Disease Patients

  • Yesim OztasEmail author
  • Selma Unal
  • Gulcin Eskandari
  • Lulufer Tamer
  • Nuriman Ozgunes
Original Article


We investigated vitamin D deficiency in pediatric sickle cell disease patients and its association with selected bone, lipid and inflammatory parameters. The study included 64 patients (33 SS and 31 SB) and 21 carriers (AS). Blood was obtained to assess levels of vitamin D, WBC, CRP, Ca, P, ALP, PTH, triglyceride, total cholesterol, LDL, VLDL, HDL, IL-2, IL-12, TNF-α, IL-4, IL-6, IL-10 and regulatory T cells. The patients were grouped according to their genotype (SS, SB) and vitamin D status (low or normal). Carriers were also grouped as low or normal vitamin D. Laboratory findings were similar between low and normal Vit D groups in SS, SB and AS genotypes except a lower IL-12 in SB-low vitamin D compared SB-normal vitamin D group. Acute chest syndrome was more frequent in SS-low Vit D (63%) compared to SS-normal Vit D (25%), SB-low Vit D (21%) and SB-normal Vit D (33%) (P = 0.045). Both SS and SB with low vitamin D had higher VLDL (P = 0.006 and P = 0.022), TNF-α (P = 0.001) and regulatory T cells (P = 0.000) compared to AS-low vitamin D. Both SS and SB with normal vitamin D had higher levels of regulatory T cells (P = 0.000) compared to AS-normal vitamin D. Vit D was not a modifier of selected inflammation, bone and lipid parameters in sickle cell disease. Acute chest syndrome was comparably more frequent in SS-low vitamin D. Increase of regulatory T cells in the patients was a result of chronic inflammation in sickle cell disease.


Sickle cell disease Genotype Vitamin D Cytokine TNF-α Regulatory T cell Lipid VLDL 



This study was funded by a grant from Turkish Scientific Research and Technical Council (112S243).

Compliance with Ethical Standards

Conflict of interest

Authors YO, SU, GE, LT and NO declare that they have no conflict of interest.

Research Involving Human Participants and/or Animals

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed Consent

Informed consent was obtained from all individual children and their parents.


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Copyright information

© Indian Society of Hematology and Blood Transfusion 2017

Authors and Affiliations

  1. 1.Department of Medical Biochemistry, Medical FacultyHacettepe UniversityAnkaraTurkey
  2. 2.Department of Pediatric Hematology, Medical FacultyMersin UniversityMersinTurkey
  3. 3.Department of Medical Biochemistry, Medical FacultyMersin UniversityMersinTurkey

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