Diego (DI) blood group genotyping is clinically important in Asian populations. Data of Diego blood type among southern Thais is still unknown. This study aimed to report DI*A and DI*B allele frequencies in southern Thai blood donors and to estimate potential risk of Dia incompatibility and alloimmunization in Thai populations. DNA samples obtained from 427 southern Thai blood donors were genotyped for DI*A and DI*B alleles by polymerase chain reaction with sequence-specific primer. DI*A and DI*B allele frequencies among southern Thais were 0.0047 and 0.9953. Their frequencies were similar to those among American Native, Italian, Filipino, Alaska Native/Aleut and Hawaiian/Pacific Islander populations; while, the frequencies significantly differed from central and northern Thai, Southeast Asian, Brazilian, Southern Brazilian, Brazilian Japanese descendants, Japanese, Han Chinese, Chinese, and Korean populations (P < 0.05). The Dia incompatibility among southern Thais (0.93%) was lower than among central Thais (3.49%), corresponding to a significantly lower probability of Dia alloimmunization (P < 0.05). This is the first report of DI*A and DI*B allele frequencies among southern Thais, which is beneficial for not only creating information for estimating risk of alloimmunization, but also providing antigen-negative red cell donors to prevent both alloimmunization and adverse transfusion reactions.
Diego blood group Genotyping Southern Thais Allele frequencies
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This work was supported by Grants of the National Research Council of Thailand and Thammasat University.
Compliance with Ethical Standards
Conflict of interest
The authors who have taken part in this study declare that they do not have anything to disclose regarding the conflict of interest with respect to this manuscript.
This study was approved by the Research Ethics Committee, National Blood Centre, Thai Red Cross Society and the Committee on Human Rights Related to Research Involving Human Subjects, Thammasat University, Pathumtani, Thailand.
Informed consent was obtained from all individual participants included in this study.
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