Effect of Nitric Oxide Donor on Metabolism of Apheresis Platelets

  • Lu Yu
  • Shifang Yu
  • Yunlei He
  • Qiming Li
  • Deyi Xu
  • Kai Huang
  • Gang Deng
  • Qiang LiEmail author
Original Article


The aim of this study is to investigate the effects of a nitric oxide (NO) donor S-nitrosoglutathione (GSNO) on the metabolism and improvement of preservation quality in apheresis platelets. A GSNO solution with a certain concentration was added into fresh apheresis platelets, and the parameters associated with platelet morphology, metabolism and function were temporally monitored for 7 days. The results showed that the NO level in GSNO group were remarkably higher than those in the control group during the whole storage stage. No significant morphology or function difference was observed between the control and GSNO groups such as Platelet count, platelet distribution width, mean platelet volume and mitochondrial metabolic activity. But in metabolism there are something differences from morphology data: pCO2, pO2, cHCO3 were also found to have no clear difference between the control and GSNO groups; the lactic acid content, sugar consumption and Lactate dehydrogenase activity in the GSNO group were lower than that in the control group at some time point; and pH values in the GSNO group were higher than the control group. Our study discovered that the NO donor GSNO can reduce the metabolism and maintain the cellular characteristics of platelets in vitro during the platelet storage period.


GSNO Nitric oxide donor Platelet storage lesion 



We thank Yan Wang and Huihui Feng for contribution to serve the blood donors and PCs collection. This work was supported by the Grants from the Project of Science and Technology Department of Ningbo (No. 2017C50046) and Project of Health Department of Ningbo (No. 2016A17).

Author’s Contributions

QL and GD conceived and designed the experiments; LY, SY, YH and GD performed the experiments and evaluated the results; QL, QL, DX and KH contributed reagents and materials; GD, LY and SY wrote the manuscript.

Compliance with Ethical Standards

Conflict of interest

None of the authors have any conflict of interest to declare.


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Copyright information

© Indian Society of Haematology & Transfusion Medicine 2017

Authors and Affiliations

  • Lu Yu
    • 1
  • Shifang Yu
    • 2
  • Yunlei He
    • 1
  • Qiming Li
    • 3
  • Deyi Xu
    • 1
  • Kai Huang
    • 1
  • Gang Deng
    • 1
  • Qiang Li
    • 4
    Email author
  1. 1.The Ningbo Central Blood StationNingboChina
  2. 2.The Department of Transfusion MedicineThe Second Affiliated Hospital of Zhejiang University School of MedicineHangzhouChina
  3. 3.The Department of CardiologyThe Fourth Affiliated Hospital of Zhejiang University School of MedicineYiwuChina
  4. 4.The Department of Laboratory MedicineThe First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhouChina

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