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Breast Cancer

, Volume 26, Issue 5, pp 573–580 | Cite as

Novel tumor-infiltrating lymphocytes ultrasonography score based on ultrasonic tissue findings predicts tumor-infiltrating lymphocytes in breast cancer

  • Kayo Fukui
  • Norio MasumotoEmail author
  • Noriyuki Shiroma
  • Akiko Kanou
  • Shinsuke Sasada
  • Akiko Emi
  • Takayuki Kadoya
  • Michiya Yokozaki
  • Koji Arihiro
  • Morihito Okada
Original Article
  • 112 Downloads

Abstract

Background

The presence of tumor-infiltrating lymphocytes (TILs) is a prognostic factor for breast cancer. However, because of tumor tissue heterogeneity, an accurate and simple evaluation method is needed. We determined if preoperative characteristic ultrasonography (US) image findings are predictive of lymphocyte-predominant breast cancer (LPBC).

Methods

We evaluated 191 patients with invasive breast cancer treated by curative surgery between January 2014 and December 2017. Stromal lymphocytes in surgical pathological specimens were evaluated. Fifty-two patients with ≥ 50% stromal TILs were defined as having LPBC. Preoperative US images were examined for indicators of TILs. The US images with characteristic TILs were scored for prediction of LPBC.

Results

Shape (more lobulated), internal echo level (weaker), and posterior echoes (stronger) were predictors of LPBC and used to assign the TILs-US scores (0–7 points); the score cutoff for predicting LPBC was 4 points (sensitivity, 0.73; specificity, 0.87; accuracy, 0.83) based on the receiver operating characteristics (ROC) curves (AUC 0.88). Multivariate logistic regression analysis identified nuclear grade (NG), OR 3.4; estrogen receptor (ER), OR 5.7; human epidermal growth factor receptor type-2 (HER2), OR 4.1; and TILs-US score, OR 14.9 as LPBC predictors (all, p < 0.05). The sensitivity, specificity, and accuracy for predicting LPBC were 0.75, 0.69, and 0.71 for NG and 0.33, 0.96, and 0.79 for ER and HER2, respectively. ROC analysis showed that the diagnostic abilities of NG, ER, and HER2 were lower than that of the TILs-US score.

Conclusions

LPBC showed characteristic US imaging findings. The TILs-US score was an accurate preoperative predictor of LPBC.

Keywords

Breast cancer Tumor-infiltrating lymphocytes Lymphocyte-predominant breast cancer Ultrasonography 

Notes

Compliance with ethical standards

Conflict of interest

None of the authors has a conflict of interest related to this study and manuscript.

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Copyright information

© The Japanese Breast Cancer Society 2019

Authors and Affiliations

  1. 1.Division of Laboratory MedicineHiroshima University HospitalHiroshimaJapan
  2. 2.Department of Surgical Oncology, Research Institute for Radiation Biology and MedicineHiroshima UniversityHiroshimaJapan
  3. 3.Department of Anatomical PathologyHiroshima University HospitalHiroshimaJapan

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