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Paraflavitalea soli gen. nov., sp. nov., isolated from greenhouse soil

  • Jun Heo
  • Hang-Yeon Weon
  • Hayoung Cho
  • Seung-Beom Hong
  • Jeong-Seon Kim
  • Soo-Jin KimEmail author
  • Soon-Wo Kwon
Article
  • 23 Downloads

Abstract

A bacterial strain designated 5GH32-13T was isolated from greenhouse soil in Yongin-city, Republic of Korea. Cells were Gram-stain-negative, strictly aerobic, motile rods of two different shapes. The strain was catalase-positive and oxidase-negative. Flexirubin-like pigments were not detected. β-Carotene was produced. The strain grew in the range of 10–37°C (optimum of 28–30°C) and pH 6–8 (optimum of pH 7) and tolerated up to 1% (w/v) NaCl (optimum of 0%). According to the 16S rRNA gene sequence comparison, strain 5GH32-13T shared a sequence similarity of less than 96.0% with all validly named taxa, having the highest sequence similarity with Pseudoflavitalea soli KIS20-3T (95.8%), Pseudoflavitalea rhizosphaerae T16R-265T (95.4%), Flavitalea gansuensis JCN-23T (95.3%), Pseudobacter ginsenosidimutans Gsoil 221T (95.3%), and Flavitalea populi HY-50RT (95.2%). A phylogenetic tree showed that strain 5GH32-13T was not grouped consistently into any specific genus. Its only polyamine was homospermidine, and its major fatty acids (> 10% of total fatty acids) were iso-C15:0, iso-C17:0 3-OH, and iso-C15:1 G. The strain’s only respiratory quinone was MK-7, and its polar lipids were phosphatidylethanolamine, one unidentified phospholipid, six unidentified aminolipids and four unidentified lipids. Its DNA G + C content was 47.5 mol%. The results from chemotaxonomic, phenotypic and phylogenetic analyses indicated that strain 5GH32-13T represents a novel species of a novel genus of the family Chitinophagaceae, and the name Paraflavitalea soli gen. nov., sp. nov. is proposed. The type strain is 5GH32-13T (= KACC 17331T = JCM 33061T).

Keywords

new genus new species Paraflavitalea soli Chitinophagaceae 

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Notes

Acknowledgments

We would like to thank Professor Bernhard Schink and Professor Aharon Oren for checking the species epithet.

This study was carried out with the support (PJ013549) of the National Institute of Agricultural Sciences, Rural Development Administration, Republic of Korea.

Supplementary material

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Supplementary material, approximately 228 KB.

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Copyright information

© The Microbiological Society of Korea 2020

Authors and Affiliations

  • Jun Heo
    • 1
  • Hang-Yeon Weon
    • 1
  • Hayoung Cho
    • 1
  • Seung-Beom Hong
    • 1
  • Jeong-Seon Kim
    • 1
  • Soo-Jin Kim
    • 1
    Email author
  • Soon-Wo Kwon
    • 1
  1. 1.Agricultural Microbiology DivisionNational Institute of Agricultural Sciences, Rural Development AdministrationJeollabuk-doRepublic of Korea

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