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Identification and characterization of a novel light-induced promoter for recombinant protein production in Pleurotus ostreatus

  • Chaomin YinEmail author
  • Xiuzhi Fan
  • Kun Ma
  • Zheya Chen
  • Defang Shi
  • Fen Yao
  • Hong Gao
  • Aimin MaEmail author
Article

Abstract

A lectin gene (plectin) with a high level of expression was previously identified by comparative transcriptome analysis of Pleurotus ostreatus. In this study, we cloned a 733-bp DNA fragment from the start codon of the plectin gene. Sequence analysis showed that the plectin promoter (Plp) region contained several eukaryotic transcription factor binding motifs, such as the TATA-box, four possible CAAT-box, light responsiveness motifs and MeJA-responsiveness motifs. To determine whether the Plp promoter was a light-regulated promoter, we constructed an expression vector with the fused egfp-hph fragment under the control of the Plp promoter and transformed P. ostreatus mycelia via Agrobacterium tume-faciens. PCR and Southern blot analyses confirmed the Plp-egfp-hph fragment was integrated into the chromosomal DNA of transformants. qRT-PCR, egfp visualization, and intra-cellular egfp determination experiments showed the Plp promoter could be a light-induced promoter that may be suitable for P. ostreatus genetic engineering. This study lays the foundation for gene homologous expression in P. ostreatus.

Keywords

Pleurotus ostreatus lectin, promoter Agrobacte-rium tumefaciens light-induction 

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Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of China (No. 31801921, 31772375 & 31601806), the Natural Science Foundation of Hubei Province (No. 2018CFB188), and the Hubei Academy of Agricultural Sciences Foundation for Young Researchers (No. 2017nkyjj04).

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Copyright information

© The Microbiological Society of Korea 2019

Authors and Affiliations

  1. 1.Institute of Agro-Products Processing and Nuclear-Agricultural TechnologyHubei Academy of Agricultural SciencesWuhanP. R. China
  2. 2.National Research and Development Center for Edible Fungi Processing (Wuhan)WuhanP. R. China
  3. 3.College of Food Science and TechnologyHuazhong Agricultural UniversityWuhanP. R. China

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