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A newly characterized exopolysaccharide from Sanghuangporus sanghuang

  • Xiao-kui MaEmail author
  • Xu She
  • Eric Charles Peterson
  • Yu Zhen Wang
  • Peng Zheng
  • Hongyan Ma
  • Kexin Zhang
  • Jian LiangEmail author
Article
  • 5 Downloads

Abstract

Sanghuangporus sanghuang is a well-known pharmacodynamic and economically important edible fungus associated with mulberry (Morus spp.). A distinctly new exopolysaccharide (EPS), designated SHP-2 was obtained from S. sanghuang P0988 broth, and its structure and anti-aging prosperity were characterized. SHP-2 was found to be composed of a back-bone of →4)-β-Manp-(1→4)-α-Araf-(1→3,4)-α-Glcp(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→3,4)-α-Glcp-(1→6)-α-Galp-(1→4)-β-Manp-(1→ and five branches, including four α-D-Glcp-(1→ and one α-D-Manp-(1→SHP-2 was shown to increase antioxidant enzyme activities including catalase (CAT) and superoxide dismutase (SOD) activities, as well as trolox equivalent antioxidant (TEAC) capacity in serum of mice pre-treated with D-Gal, while reducing lipofuscin levels. SHP-2 exerted a favorable influence on immune organ coefficients and ameliorated the histopathological hepatic lesions and apoptosis in hepatocytes of Dgalactose- aged mice almost in a dose-dependent manner. Using the same analytical methods, on comparison with previously studied EPS compounds (i.e. SHP-1), SHP-2 was found to have more complex structure, larger molecule weight, and different anti-aging properties. The results presented here suggest that not only does EPS bioactivity vary with respect to molecular structures and molecule weight, but that multiple structures with different activity can be expressed by a single fungal strain. These results may help understanding the antiaging prosperity of these polysaccharides for use in health foods or dietary supplements.

Keywords

Sanghuangporus sanghuang biomacromolecule, exopolysaccharide antioxidant mammalian system 

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Notes

Acknowledgments

This work was supported by Fundamental Research Funds for the Central Universities of China (Grant No. GK201702014, GK201603110, and GK201806007) and National Key R&D Program of China (2017YFD0800200).

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Copyright information

© The Microbiological Society of Korea 2019

Authors and Affiliations

  • Xiao-kui Ma
    • 1
    Email author
  • Xu She
    • 1
  • Eric Charles Peterson
    • 2
  • Yu Zhen Wang
    • 1
  • Peng Zheng
    • 1
  • Hongyan Ma
    • 1
  • Kexin Zhang
    • 1
  • Jian Liang
    • 1
    Email author
  1. 1.Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, Ministry of Education, National Engineering Laboratory for Resource Developing of Endangered Chinese Crude Drugs in Northwest of China, College of Life ScienceShaanxi Normal UniversityShaanxiP. R. China
  2. 2.Agency for Science, Technology, and Research (A*STAR)SingaporeSingapore

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