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Isolation, cultivation, and genome analysis of proteorhodopsin-containing SAR116-clade strain Candidatus Puniceispirillum marinum IMCC1322

  • Junhak Lee
  • Kae Kyoung Kwon
  • Seung-Il Lim
  • Jaeho Song
  • Ah Reum Choi
  • Sung-Hyun Yang
  • Kwang-Hwan Jung
  • Jung-Hyun Lee
  • Sung Gyun Kang
  • Hyun-Myung OhEmail author
  • Jang-Cheon ChoEmail author
Article

Abstract

Strain IMCC1322 was isolated from a surface water sample from the East Sea of Korea. Based on 16S rRNA analysis, IMCC1322 was found to belong to the OCS28 sub-clade of SAR116. The cells appeared as short vibrioids in logarithmic-phase culture, and elongated spirals during incubation with mitomycin or in aged culture. Growth characteristics of strain IMCC1322 were further evaluated based on genomic information; proteorhodopsin (PR), carbon monoxide dehydro-genase, and dimethylsulfoniopropionate (DMSP)-utilizing enzymes. IMCC1322 PR was characterized as a functional retinylidene protein that acts as a light-driven proton pump in the cytoplasmic membrane. However, the PR-dependent phototrophic potential of strain IMCC1322 was only observed under CO-inhibited and nutrient-limited culture conditions. A DMSP-enhanced growth response was observed in addition to cultures grown on C1 compounds like methanol, formate, and methane sulfonate. Strain IMCC1322 cultivation analysis revealed biogeochemical processes characteristic of the SAR116 group, a dominant member of the microbial community in euphotic regions of the ocean. The polyphasic taxonomy of strain IMCC1322 is given as Candidatus Puniceis-pirillum marinum, and was confirmed by chemotaxonomic tests, in addition to 16S rRNA phylogeny and cultivation analyses.

Keywords

Canididatus Puniceispirillum marinum carbon monoxide dimethylsulfoniopropionate light-emitting diode mitomycin C proteorhodopsin SAR116 

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Notes

Acknowledgments

This research was supported by the Marine Biotechnology Program of the Korea Institute of Marine Science and Technology Promotion (KIMST) funded by the Ministry of Oceans and Fisheries (MOF) [No. 20180430]; and by the Korea National Research Fund [NRF-2017R1D1A1B03034706].

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Copyright information

© The Microbiological Society of Korea 2019

Authors and Affiliations

  • Junhak Lee
    • 1
    • 2
  • Kae Kyoung Kwon
    • 3
  • Seung-Il Lim
    • 4
  • Jaeho Song
    • 5
  • Ah Reum Choi
    • 6
  • Sung-Hyun Yang
    • 3
  • Kwang-Hwan Jung
    • 6
  • Jung-Hyun Lee
    • 3
  • Sung Gyun Kang
    • 3
  • Hyun-Myung Oh
    • 1
    Email author
  • Jang-Cheon Cho
    • 5
    Email author
  1. 1.Institute of Liberal Arts EducationPukyong National UniversityBusanRepublic of Korea
  2. 2.Korea Marine Equipment Research InstituteBusanRepublic of Korea
  3. 3.Marine Biotechnology Research DivisionKorea Institute of Ocean Science & TechnologyBusanRepublic of Korea
  4. 4.Cell Biotech, Co., Ltd.GimpoRepublic of Korea
  5. 5.Division of Biology and Ocean SciencesInha UniversityIncheonRepublic of Korea
  6. 6.Department of Life Science and Interdisciplinary Program of Integrated BiotechnologySogang UniversitySeoulRepublic of Korea

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