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Journal of Microbiology

, Volume 57, Issue 2, pp 138–142 | Cite as

Oxygen-mediated growth enhancement of an obligate anaerobic archaeon Thermococcus onnurineus NA1

  • Seong Hyuk Lee
  • Hwan Youn
  • Sung Gyun KangEmail author
  • Hyun Sook LeeEmail author
Microbial Physiology and Biochemistry

Abstract

Thermococcus onnurineus NA1, an obligate anaerobic hyperthermophilic archaeon, showed variable oxygen (O2) sensitivity depending on the types of substrate employed as an energy source. Unexpectedly, the culture with yeast extract as a sole energy source showed enhanced growth by 2-fold in the presence of O2. Genome-wide transcriptome analysis revealed the upregulation of several antioxidant-related genes encoding thioredoxin peroxidase (TON_0862), rubrerythrin (TON_0864), rubrerythrin-related protein (TON_0873), NAD(P)H rubredoxin oxidoreductase (TON_0865), or thioredoxin reductase (TON_1603), which can couple the detoxification of reactive oxygen species with the regeneration of NAD(P)+ from NAD(P)H. We present a plausible mechanism by which O2 serves to maintain the intracellular redox balance. This study demonstrates an unusual strategy of an obligate anaerobe underlying O2-mediated growth enhancement despite not having heme-based or cytochrome-type proteins.

Keywords

oxygen obligate anaerobic archaeon Thermococcus onnurineus NA1 transcriptome analysis antioxidant enzymes 

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Supplementary material

12275_2019_8592_MOESM1_ESM.xlsx (54 kb)
Supplementary data Table S1. COG functional classification of downregulated genes by 20% O2
12275_2019_8592_MOESM2_ESM.xlsx (39 kb)
Supplementary data Table S2. COG functional classification of upregulated genes by 20% O2

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Copyright information

© The Microbiological Society of Korea and Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Korea Institute of Ocean Science and TechnologyBusanRepublic of Korea
  2. 2.Department of BiologyCalifornia State UniversityFresnoUSA
  3. 3.Department of Marine BiotechnologyKorea University of Science and TechnologyDaejeonRepublic of Korea

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