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Journal of Microbiology

, Volume 57, Issue 2, pp 113–121 | Cite as

Rotavirus-mediated alteration of gut microbiota and its correlation with physiological characteristics in neonatal calves

  • Ja-Young Jang
  • Suhee Kim
  • Min-Sung Kwon
  • Jieun Lee
  • Do-Hyeon Yu
  • Ru-Hui Song
  • Hak-Jong ChoiEmail author
  • Jinho ParkEmail author
Microbial Systematics and Evolutionary Microbiology

Abstract

Diarrhea is a fatal disease to neonatal calves, and rotavirus is the main pathogen associated with neonatal calf diarrhea. Although previous studies have reported that the gut microbiota is changed in calves during diarrhea, less is known about whether rotavirus infection alters the structure of the gut microbiota. Here, we characterized fecal microbial communities and identified possible relationships between the gut microbiota profiles and physiological parameters. Five fecal specimens of rotavirus-infected calves from 1 to 30 days after birth and five fecal specimens of age-matched healthy calves were used for the microbial community analysis using the Illumina MiSeq sequencer. Rotavirus infection was associated with reduced rotavirus infection significantly reduced the richness and diversity of the bacterial community. Weighted unique fraction metric analysis exhibited significant differences in community membership and structure between healthy and rotavirus-infected calves. Based on relative abundance analysis and linear discriminant analysis effect size, we found that the representative genera from Lactobacillus, Subdoligranulum, Blautia, and Bacteroides were closely related to healthy calves, while the genera Escherichia and Clostridium were closely affiliated to rotavirus-infected calves. Furthermore, canonical correlation analysis and Pearson correlation coefficient results revealed that the increased relative abundances of Lactobacillus, Subdoligranulum, and Bacteroides were correlated with normal levels of physiological characteristics such as white blood cells, blood urea nitrogen, serum amyloid protein A, and glucose concentration in serum. These results suggest that rotavirus infection alters the structure of the gut microbiota, correlating changes in physiological parameters. This study provides new information on the relationship between gut microbiota and the physiological parameters of rotavirus-mediated diarrheic calves.

Keywords

diarrhea rotavirus gut microbiota physiological parameters 

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References

  1. Ammar, S.S., Mokhtaria, K., Tahar, B.B., Amar, A.A., Redha, B.A., Yuva, B., Mohamed, H.S., Abdellatif, N., and Laid, B. 2014. Prevalence of rotavirus (GARV) and coronavirus (BCoV) associated with neonatal diarrhea in calves in western Algeria. Asian Pac. J. Trop. Biomed. 4, S318–S322.CrossRefGoogle Scholar
  2. Cho, Y.I., Kim, W.I., Liu, S., Kinyon, J.M., and Yoon, K.J. 2010. Development of a panel of multiplex real-time polymerase chain reaction assays for simultaneous detection of major agents causing calf diarrhea in feces. J. Vet. Diagn. Invest. 22, 509–517.CrossRefGoogle Scholar
  3. Cho, Y.I. and Yoon, K.J. 2014. An overview of calf diarrhea - infectious etiology, diagnosis, and intervention. J. Vet. Sci. 15, 1–17.CrossRefGoogle Scholar
  4. Edrington, T.S., Dowd, S.E., Farrow, R.F., Hagevoort, G.R., Callaway, T.R., Anderson, R.C., and Nisbet, D.J. 2012. Development of colonic microflora as assessed by pyrosequencing in dairy calves fed waste milk. J. Dairy Sci. 95, 4519–4525.CrossRefGoogle Scholar
  5. Gomez, D.E., Arroyo, L.G., Costa, M.C., Viel, L., and Weese, J.S. 2017. Characterization of the fecal bacterial microbiota of healthy and diarrheic dairy calves. J. Vet. Intern. Med. 31, 928–939.CrossRefGoogle Scholar
  6. Gulliksen, S.M., Jor, E., Lie, K.I., Hamnes, I.S., Loken, T., Akerstedt, J., and Osteras, O. 2009. Enteropathogens and risk factors for diarrhea in Norwegian dairy calves. J. Dairy Sci. 92, 5057–5066.CrossRefGoogle Scholar
  7. Hur, T.Y., Jung, Y.H., Choe, C.Y., Cho, Y.I., Kang, S.J., Lee, H.J., Ki, K.S., Baek, K.S., and Suh, G.H. 2013. The dairy calf mortality: the causes of calf death during ten years at a large dairy farm in Korea. Korean J. Vet. Res. 53, 103–108.CrossRefGoogle Scholar
  8. Izzo, M.M., Kirkland, P.D., Mohler, V.L., Perkins, N.R., Gunn, A.A., and House, J.K. 2011. Prevalence of major enteric pathogens in Australian dairy calves with diarrhoea. Aust. Vet. J. 89, 167–173.CrossRefGoogle Scholar
  9. Kapikian, A.Z. and Chanock, R.M. 1996. Rotaviruses, pp. 1657–1708. In Straus, S.E. (ed.), Fields Virology Vol. 2. Lippincott-Raven, Philadelphia, USA.Google Scholar
  10. Klein-Jobstl, D., Schornsteiner, E., Mann, E., Wagner, M., Drillich, M., and Schmitz-Esser, S. 2014. Pyrosequencing reveals diverse fecal microbiota in simmental calves during early development. Front. Microbiol. 5, 622.Google Scholar
  11. Kostic, A.D., Xavier, R.J., and Gevers, D. 2014. The microbiome in inflammatory bowel disease: current status and the future ahead. Gastroenterology 146, 1489–1499.CrossRefGoogle Scholar
  12. Li, R.W., Connor, E.E., Li, C., Baldwin Vi, R.L., and Sparks, M.E. 2012. Characterization of the rumen microbiota of pre-ruminant calves using metagenomic tools. Environ. Microbiol. 14, 129–139.CrossRefGoogle Scholar
  13. Lukas, F., Koppova, I., Kudrna, V., and Kopecny, J. 2007. Postnatal development of bacterial population in the gastrointestinal tract of calves. Folia Microbiol. 52, 99–104.CrossRefGoogle Scholar
  14. Margreiter, M., Ludl, K., Phleps, W., and Kaehler, S.T. 2006. Therapeutic value of a Lactobacillus gasseri and Bifidobacterium longum fixed bacterium combination in acute diarrhea: a randomized, double-blind, controlled clinical trial. Int. J. Clin. Pharmacol. Ther. 44, 207–215.CrossRefGoogle Scholar
  15. Millar, H.R., Simpson, J.G., and Stalker, A.L. 1971. An evaluation of the heat precipitation method for plasma fibrinogen estimation. J. Clin. Pathol. 24, 827–830.CrossRefGoogle Scholar
  16. Oikonomou, G., Teixeira, A.G., Foditsch, C., Bicalho, M.L., Machado, V.S., and Bicalho, R.C. 2013. Fecal microbial diversity in preweaned dairy calves as described by pyrosequencing of metagenomic 16S rDNA. Associations of Faecalibacterium species with health and growth. PLoS One 8, e63157.CrossRefGoogle Scholar
  17. Penders, J., Thijs, C., Vink, C., Stelma, F.F., Snijders, B., Kummeling, I., van den Brandt, P.A., and Stobberingh, E.E. 2006. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 118, 511–521.CrossRefGoogle Scholar
  18. Rolhion, N. and Chassaing, B. 2016. When pathogenic bacteria meet the intestinal microbiota. Philos. Trans. R. Soc. Lond. B Biol. Sci. 371, 20150504.CrossRefGoogle Scholar
  19. Singh, P., Teal, T.K., Marsh, T.L., Tiedje, J.M., Mosci, R., Jernigan, K., Zell, A., Newton, D.W., Salimnia, H., Lephart, P., et al. 2015. Intestinal microbial communities associated with acute enteric infections and disease recovery. Microbiome 3, 45.CrossRefGoogle Scholar
  20. Uetake, K. 2013. Newborn calf welfare: a review focusing on mortality rates. Animal Sci. J. 84, 101–105.CrossRefGoogle Scholar
  21. USDA. 2008. Dairy 2007 Part II: changes in the US dairy cattle industry, 1991–2007. Fort Collins: USDA-APHIS-VS, CEAH, 57–61.Google Scholar
  22. Uyeno, Y., Sekiguchi, Y., and Kamagata, Y. 2010. rRNA-based analysis to monitor succession of faecal bacterial communities in Holstein calves. Lett. Appl. Microbiol. 51, 570–577.CrossRefGoogle Scholar
  23. Wotzka, S.Y., Nguyen, B.D., and Hardt, W.D. 2017. Salmonella Typhimurium diarrhea reveals basic principles of enteropathogen infection and disease-promoted DNA exchange. Cell Host Microbe 21, 443–454.CrossRefGoogle Scholar

Copyright information

© The Microbiological Society of Korea and Springer Nature B.V. 2019

Authors and Affiliations

  • Ja-Young Jang
    • 1
  • Suhee Kim
    • 2
  • Min-Sung Kwon
    • 1
  • Jieun Lee
    • 1
  • Do-Hyeon Yu
    • 3
  • Ru-Hui Song
    • 4
  • Hak-Jong Choi
    • 1
    Email author
  • Jinho Park
    • 4
    Email author
  1. 1.Research and Development DivisionWorld Institute of KimchiGwangjuRepublic of Korea
  2. 2.Division of Animal Diseases and Health, National Institute of Animal ScienceRural Development AdministrationWanjuRepublic of Korea
  3. 3.Institute of Animal Medicine, College of Veterinary MedicineGyeongsang National UniversityJinjuRepublic of Korea
  4. 4.Department of Veterinary Internal Medicine, College of Veterinary MedicineChonbuk National UniversityIksanRepublic of Korea

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