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Journal of Microbiology

, Volume 57, Issue 1, pp 64–73 | Cite as

Effective mucosal live attenuated Salmonella vaccine by deleting phosphotransferase system component genes ptsI and crr

  • Yong Zhi
  • Shun Mei Lin
  • A-Yeung Jang
  • Ki Bum Ahn
  • Hyun Jung Ji
  • Hui-Chen Guo
  • Sangyong LimEmail author
  • Ho Seong SeoEmail author
Microbial Pathogenesis and Host-Microbe Interaction
  • 30 Downloads

Abstract

Salmonella enterica is a major human pathogen that causes invasive non-typhoidal Salmonellosis (iNTS), resulting in significant morbidity and mortality. Although a number of pre-clinical and clinical studies have reported on the feasibility of developing a safe and effective vaccine against iNTS, there have been no licensed Salmonella vaccines available to protect against NTS strains. Vaccine formulations of highest priority for NTS are live attenuated vaccines, which can elicit effective induction of intestinal mucosal and intracellular bacteria-specific cell mediated immune responses. Since glucose is crucial for intracellular survival and replication in host cells, we constructed strains with mutations in components of the glucose uptake system, called the phosphotransferase system (PTS), and compared the relative virulence and immune responses in mice. In this study, we found that the strain with mutations in both ptsI and crr (KST0556) was the most attenuated strain among the tested strains, and proved to be highly effective in inducing a mucosal immune response that can protect against NTS infections in mice. Thus, we suggest here that KST0556 (Δptscrr) is a potential live vaccine candidate for NTS, and may also be a candidate for a live delivery vector for heterologous antigens. Moreover, since PTS is a well-conserved glucose transporter system in both Gramnegative and Gram-positive bacteria, the ptsI and crr genes may be potential targets for creating live bacterial vectors or vaccine strains.

Keywords

Salmonella Typhimurium phosphotransferase system live attenuation vaccine 

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Copyright information

© The Microbiological Society of Korea and Springer Nature B.V. 2019

Authors and Affiliations

  • Yong Zhi
    • 1
    • 2
  • Shun Mei Lin
    • 1
  • A-Yeung Jang
    • 1
    • 3
  • Ki Bum Ahn
    • 1
  • Hyun Jung Ji
    • 1
    • 4
  • Hui-Chen Guo
    • 5
  • Sangyong Lim
    • 1
    • 2
    Email author
  • Ho Seong Seo
    • 1
    • 2
    Email author
  1. 1.Radiation Biotechnology DivisionKorea Atomic Energy Research InstituteJeongeupRepublic of Korea
  2. 2.Department of Radiation Biotechnology and Applied Radioisotope ScienceUniversity of Science and TechnologyDaejeonRepublic of Korea
  3. 3.Department of Biological SciencesChonbuk National UniversityJeonjuRepublic of Korea
  4. 4.Department of Oral Microbiology and Immunology, DRI, and BK21 Plus Program, School of DentistrySeoul National UniversitySeoulRepublic of Korea
  5. 5.State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhouP. R. China

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