Journal of Microbiology

, Volume 56, Issue 4, pp 255–263 | Cite as

Proteome analysis reveals global response to deletion of mrflbA in Monascus ruber

  • Qingqing Yan
  • Zhouwei Zhang
  • Yishan Yang
  • Fusheng Chen
  • Yanchun Shao
Microbial Physiology and Biochemistry
  • 54 Downloads

Abstract

Monascus spp. are commonly used for a wide variety of applications in the food and pharmaceutical industries. In previous studies, the knock-out of mrflbA (a putative regulator of the G protein α subunit) in M. ruber led to autolysis of the mycelia, decreased pigmentation and lowered mycotoxin production. Therefore, we aimed to obtain a comprehensive overview of the underlying mechanism of mrflbA deletion at the proteome level. A two-dimensional gel electrophoresis analysis of mycelial proteins indicated that the abundance of 178 proteins was altered in the ΔmrflbA strain, 33 of which were identified with high confidence. The identified proteins are involved in a range of activities, including carbohydrate and amino acid metabolism, hyphal development and the oxidative stress response, protein modification, and the regulation of cell signaling. Consistent with these findings, the activity of antioxidative enzymes and chitinase was elevated in the supernatant of the ΔmrflbA strain. Furthermore, deletion of mrflbA resulted in the transcriptional reduction of secondary metabolites (pigment and mycotoxin). In short, the mutant phenotypes induced by the deletion of mrflbA were consistent with changes in the expression levels of associated proteins, providing direct evidence of the regulatory functions mediated by mrflbA in M. ruber.

Keywords

Monasucs ruber comparative proteomics development modulation regulator of G protein α subunit secondary metabolites 

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Supplementary material

12275_2018_7425_MOESM1_ESM.pdf (355 kb)
Supplementary data Table S1. All identified proteins with differential expression in mrflbA-deletion strain and the wild type

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Copyright information

© The Microbiological Society of Korea and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Qingqing Yan
    • 1
  • Zhouwei Zhang
    • 2
  • Yishan Yang
    • 1
  • Fusheng Chen
    • 1
  • Yanchun Shao
    • 1
  1. 1.College of Food Science and TechnologyHuazhong Agricultural UniversityWuhanP. R. China
  2. 2.Institute of Agricultural Products Processing and Nuclear Agricultural TechnologyHubei Academy of Agricultural ScienceWuhanP. R. China

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