Multimodal bioimaging based on gold nanorod and carbon dot nanohybrids as a novel tool for atherosclerosis detection
- 444 Downloads
Advanced biocompatible and robust platforms equipped with diverse properties are highly required in biomedical imaging applications for the early detection of atherosclerotic vascular disease and cancers. Designing nanohybrids composed of noble metals and fluorescent materials is a new way to perform multimodal imaging to overcome the limitations of single-modality counterparts. Herein, we propose the novel design of a multimodal contrast agent; namely, an enhanced nanohybrid comprising gold nanorods (GNRs) and carbon dots (CDs) with silica (SiO2) as a bridge. The nanohybrid (GNR@SiO2@CD) construction is based on covalent bonding between SiO2 and the silane-functionalized CDs, which links the GNRs with the CDs to form typical core–shell units. The novel structure not only retains and even highly improves the optical properties of the GNRs and CDs, but also possesses superior imaging performance in both diffusion reflection (DR) and fluorescence lifetime imaging microscopy (FLIM) measurements compared with bare GNRs or fluorescence dyes and CDs. The superior bioimaging properties of the GNR@SiO2@CD nanohybrids were successfully exploited for in vitro DR and FLIM measurements of macrophages within tissue-like phantoms, paving the way toward a theranostic contrast agent for atherosclerosis and cancer.
Keywordsnanohybrids multimodal imaging contrast agent diffusion reflection fluorescence lifetime imaging atherosclerosis macrophages
Unable to display preview. Download preview PDF.
This work was supported by the Joint NSFC-ISF Research Program (No. 51561145004), jointly funded by the National Natural Science Foundation of China and the Israel Science Foundation, and the President’s International Fellowship Initiative, Chinese Academy of Sciences (No. PIFI2015VTB041).
- Ankri, R.; Leshem-Lev, D.; Fixler, D.; Popovtzer, R.; Motiei, M.; Kornowski, R.; Hochhauser, E.; Lev, E. I. Gold nanorods as absorption contrast agents for the noninvasive detection of arterial vascular disorders based on diffusion reflection measurements. Nano Lett. 2014, 14, 2681–2687.CrossRefGoogle Scholar
- Ankri, R.; Ashkenazy, A.; Milstein, Y.; Brami, Y.; Olshinka, A.; Goldenberg-Cohen, N.; Popovtzer, A.; Fixler, D.; Hirshberg, A. Gold nanorods based air scanning electron microscopy and diffusion reflection imaging for mapping tumor margins in squamous cell carcinoma. ACS Nano 2016, 10, 2349–2356.CrossRefGoogle Scholar
- Ankri, R.; Melzer, S.; Tarnok, A.; Fixler, D. Detection of gold nanorods uptake by macrophages using scattering analyses combined with diffusion reflection measurements as a potential tool for in vivo atherosclerosis tracking. Int. J. Nanomed. 2015, 10, 4437–4446.Google Scholar
- Ouhenia-Ouadahi, K.; Yasukuni, R.; Yu, P.; Laurent, G.; Pavageau, C.; Grand, J.; Guérin, J.; Léaustic, A.; Félidj, N.; Aubard, J. et al. Photochromic–fluorescent–plasmonic nanomaterials: Towards integrated three-component photoactive hybrid nanosystems. Chem. Commun. 2014, 50, 7299–7302.CrossRefGoogle Scholar
- Lai, C. W.; Hsiao, J. K.; Chen, Y. C.; Chou, P. T. Spherical and anisotropic silica shell nanomaterials. In Nanotechnologies for the Life Sciences. Kumar, C., Ed.; Wiley-VCH Verlag GmbH & Co: Weinheim, 2009.Google Scholar
- Liu, Y. L.; Yang, M.; Zhang, J. P.; Zhi, X.; Li, C.; Zhang, C. L.; Pan, F.; Wang, K.; Yang, Y. M.; de la Fuentea, J. M. et al. Martinez de la Fuentea, J.Human induced pluripotent stem cells for tumor targeted delivery of gold nanorods and enhanced photothermal therapy. ACS Nano. 2016, 10, 2375–2385.CrossRefGoogle Scholar