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Green synthesis and biological activities of silver nanoparticles prepared by Carpesium cernuum extract

  • Eun-Young Ahn
  • Hang Jin
  • Youmie ParkEmail author
Research Article
  • 60 Downloads

Abstract

The extract of Carpesium cernuum whole plant was successfully used as a green factory for the synthesis of silver nanoparticles in a one-step, one-pot process. The extract efficiently reduced silver ions to spherical silver nanoparticles. The size was measured as 13.0 ± 0.2 nm from high resolution transmission electron microscopic images. The reaction yield was determined to be 99.6% using inductively coupled plasma optical emission spectroscopy. The silver nanoparticles were highly stable for 28 days at ambient temperature without forming agglomeration or aggregation of nanoparticles. Dose-dependent antioxidant activity of the silver nanoparticles was observed in terms of the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl radicals. The silver nanoparticles also exerted cytotoxicity on Mus musculus skin melanoma cells (B16F10) and human lung cancer cells (A549) in a dose-dependent manner. Specifically, the cytotoxicity of the silver nanoparticles on A549 cells was closely associated with apoptotic cell death. Cellular uptake of the silver was evaluated via inductively coupled plasma mass spectrometry, and a higher percentage of silver was taken up by A549 cells (22.6%) than by B16F10 cells (17.3%). This result indicated that higher cellular uptake of silver nanoparticles resulted in higher cytotoxicity on A549 cells. Therefore, plant extracts are capable of being valuable natural sources for the green synthesis of silver nanoparticles that exhibit potent biological activities for pharmaceutical and biomedical applications in future nanomedicine.

Keywords

Silver nanoparticles Carpesium cernuum Green synthesis Antioxidant activity Cytotoxicity 

Notes

Acknowledgements

This work was supported by a grant from Inje University for the research in 2018 (Grant No. 20180019). The authors would like to thank Ms. Inyoung Cho at Seoul National University for editing assistance.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

References

  1. Akter M, Sikder MT, Rahman MM, Ullah AKMA, Hossain KFB, Banik S, Hosokawa T, Saito T, Kurasaki M (2018) A systematic review on silver nanoparticles-induced cytotoxicity: physicochemical properties and perspectives. J Adv Res 9:1–16CrossRefGoogle Scholar
  2. Cameron SJ, Hosseinian F, Willmore WG (2018) A current overview of the biological and cellular effects of nanosilver. Int J Mol Sci 12:2030CrossRefGoogle Scholar
  3. Choi Y, Choi MJ, Cha SH, Kim YS, Cho S, Park Y (2014) Catechin-capped gold nanoparticles: green synthesis, characterization, and catalytic activity toward 4-nitrophenol reduction. Nanoscale Res Lett 9:103CrossRefGoogle Scholar
  4. Choi Y, Kang S, Cha SH, Kim HS, Song K, Lee YJ, Kim K, Kim YS, Cho S, Park Y (2018) Platycodon saponins from platycodi radix (Platycodon grandiflorum) for the green synthesis of gold and silver nanoparticles. Nanoscale Res Lett 13:23CrossRefGoogle Scholar
  5. De Matteis V, Cascione M, Toma CC, Leporatti S (2018) Silver nanoparticles: synthetic routes, in vitro toxicity and theranostic applications for cancer disease. Nanomaterials (Basel) 8:319CrossRefGoogle Scholar
  6. Hanan NA, Chiu HI, Ramachandran MR, Tung WH, Mohamad Zain NN, Yahaya N, Lim V (2018) Cytotoxicity of plant-mediated synthesis of metallic nanoparticles: a systematic review. Int J Mol Sci 19:1725CrossRefGoogle Scholar
  7. Hembram KC, Kumar R, Kandha L, Parhi PK, Kundu CN, Bindhani BK (2018) Therapeutic prospective of plant-induced silver nanoparticles: application as antimicrobial and anticancer agent. Artif Cells Nanomed Biotechnol 46:S38–S51CrossRefGoogle Scholar
  8. Khan M, Shaik MR, Adil SF, Khan ST, Al-Warthan A, Siddiqui MRH, Tahir MN, Tremel W (2018) Plant extracts as green reductants for the synthesis of silver nanoparticles: lessons from chemical synthesis. Dalton Trans 47:11988–12010CrossRefGoogle Scholar
  9. Kim TY, Cha SH, Cho S, Park Y (2016) Tannic acid-mediated green synthesis of antibacterial silver nanoparticles. Arch Pharm Res 39:465–473CrossRefGoogle Scholar
  10. Koduru JR, Kailasa SK, Bhamore JR, Kim KH, Dutta T, Vellingiri K (2018) Phytochemical-assisted synthetic approaches for silver nanoparticles antimicrobial applications: a review. Adv Colloid Interface Sci 256:326–339CrossRefGoogle Scholar
  11. Lim SH, Park Y (2018) Green synthesis, characterization and catalytic activity of gold nanoparticles prepared usig rosmarinic acid. J Nanosci Nanotechnol 18:659–667CrossRefGoogle Scholar
  12. Liu QX, Yang YX, Zhang JP, Chen LP, Shen YH, Li HL, Zhang WD (2016) Isolation, structure elucidation, and absolute configuration of highly oxygenated germacranolides from Carpesium cernuum. J Nat Prod 79:2479–2486CrossRefGoogle Scholar
  13. Ma JP, Tan CH, Zhu DY (2008) Glycosidic constituents from Carpesium cernuum L. J Asian Nat Prod Res 10:565–569CrossRefGoogle Scholar
  14. Marin S, Vlasceanu GM, Tiplea RE, Bucur IR, Lemnaru M, Marin MM, Grumezescu AM (2015) Applications and toxicity of silver nanoparticles: a recent review. Curr Top Med Chem 15:1596–1604CrossRefGoogle Scholar
  15. Ovais M, Ahmad I, Khalil AT, Mukherjee S, Javed R, Ayaz M, Raza A, Shinwari ZK (2018) Wound healing applications of biogenic colloidal silver and gold nanoparticles: recent trends and future prospects. Appl Microbiol Biotechnol 102:4305–4318CrossRefGoogle Scholar
  16. Park Y, Hong YN, Weyers A, Kim YS, Linhardt RJ (2011) Polysaccharides and phytochemicals: a natural reservoir for the green synthesis of gold and silver nanoparticles. IET Nanobiotechnol 5:69–78CrossRefGoogle Scholar
  17. Park S, Cha SH, Cho I, Park S, Park Y, Cho S, Park Y (2016) Antibacterial nanocarriers of resveratrol with gold and silver nanoparticles. Mater Sci Eng C 58:1160–1169CrossRefGoogle Scholar
  18. Park JS, Ahn EY, Park Y (2017) Asymmetric dumbbell-shaped silver nanoparticles and spherical gold nanoparticles green-synthesized by mangosteen (Garcinia mangostana) pericarp waste extracts. Int J Nanomed 12:6895–6908CrossRefGoogle Scholar
  19. Rai MK, Deshmukh SD, Ingle AP, Gade AK (2012) Silver nanoparticles: the powerful nanoweapon against multidrug-resistant bacteria. J Appl Microbiol 112:841–852CrossRefGoogle Scholar
  20. Ramasamy T, Ruttala HB, Kanu BG, Poudel BK, Choi HG, Yong CS, Kim JO (2017) Smart chemistry-based nanosized drug delivery systems for systemic applications: a comprehensive review. J Control Release 258:226–253CrossRefGoogle Scholar
  21. Ramasamy T, Ruttala HB, Sundaramoorthy P, Poudel BK, Youn YS, Ku SK, Choi HG, Yong CS, Kim JO (2018) Multimodal selenium nanoshell-capped Au@mSiO2 nanoplatform for NIR responsive chemo-photothermal therapy against metastatic breast cancer. NPG Asia Mater 10:197–216CrossRefGoogle Scholar
  22. Ruttala HB, Ramasamy T, Madeshwaran T, Hiep TT, Kandasamy U, Oh KT, Choi HG, Yong CS, Kim JO (2018) Emerging potential of stimulus-responsive nanosized anticancer drug delivery systems for systemic applications. Arch Pharm Res 41:111–129CrossRefGoogle Scholar
  23. Saratale RG, Karuppusamy I, Saratale GD, Pugazhendhi A, Kumar G, Park Y, Ghodake GS, Bharagava RN, Banu JR, Shin HS (2018) A comprehensive review on green nanomaterials using biological systems: recent perception and their future applications. Colloids Surf B 170:20–35CrossRefGoogle Scholar
  24. Wei L, Lu J, Xu H, Patel A, Chen ZS, Chen G (2015) Silver nanoparticles: synthesis, properties, and therapeutic applications. Drug Discov Today 20:595–601CrossRefGoogle Scholar
  25. Yadi M, Mostafavi E, Saleh B, Davaran S, Aliyeva I, Khalilov R, Nikzamir M, Nikzamir N, Akbarzadeh A, Panahi Y, Milani M (2018) Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artif Cells Nanomed Biotechnol 46:S336–S343CrossRefGoogle Scholar
  26. Yang C, Zhu QX, Zhang Q, Wang Q, Jia ZJ (2001) Eudesmanolides, aromatic derivatives, and other constituents from Carpesium cernuum. Pharmazie 56:825–827Google Scholar
  27. Zhang XF, Shen W, Gurunathan S (2016) Silver nanoparticle-mediated cellular responses in various cell lines: an in vitro model. Int J Mol Sci 17:1603CrossRefGoogle Scholar

Copyright information

© The Pharmaceutical Society of Korea 2019

Authors and Affiliations

  1. 1.College of Pharmacy and Inje Institute of Pharmaceutical Sciences and Research, Inje UniversityGimhaeRepublic of Korea
  2. 2.Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences (YAAS)KunmingChina

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