Lignans from Saururus chinensis exhibit anti-inflammatory activity by influencing the Nrf2/HO-1 activation pathway
- 143 Downloads
As part of our ongoing program to develop anti-inflammatory agents, an extract derived from Saururus chinensis collected in Korea was found to inhibit nitric oxide (NO) production in RAW264.7 cells. Bioassay-guided fractionation led to the isolation two new (1 and 2) and six known dineolignans (3–8). To the best of our knowledge, manassatin B1 (3) was isolated from S. chinensis for the first time. All structures were elucidated using extensive spectroscopic analysis. Of these compounds, 2 and 8 inhibited lipopolysaccharide (LPS)-induced production of NO and showed IC50 values of 5.80 and 1.52 µM, respectively. LPS-induced expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) was also significantly suppressed by the administration of 2 and 8. In addition, these lignans induced the expression of heme oxygenase-1 (HO-1) in a concentration-dependent manner. Nuclear translocation of nuclear-E2-related factor 2 (Nrf2), a key regulator of HO-1 protein expression, was also induced in RAW264.7 cells treated with 2 and 8. These findings suggested that these lignans exerted anti-inflammatory effects in RAW264.7 cells through modulation of the Nrf2/HO-1 pathway and that they were potential HO-1 inducers for preventing or treating inflammation.
KeywordsSaururus chinensis Saururaceae Lignan Anti-inflammatory Nrf2 HO-1
This research was supported by the National Research Foundation of Korea (NRF-2016R1D1A1B03930246) by the Ministry of Education, Korea. We are grateful to the Korea Basic Science Institute (KBSI) for mass spectrometric measurements.
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
- Hai Dang N, Choo YY, Tien Dat N, Hoai Nam N, Van Minh C, Lee JH (2016) 7-Methoxy-(9H-β-carbolin-1-il)-(E)-1-propenoic acid, a β-carboline alkaloid from Eurycoma longifolia, exhibits anti-inflammatory effects by activating the Nrf2/Heme oxygenase-1 pathway. J Cell Biochem 117:659–670CrossRefGoogle Scholar
- Lee D-S, Kim KS, Ko W, Li B, Keo S, Jeong GS, Oh H, Kim YC (2014) The neoflavonoid latifolin isolated from MeOH extract of Dalbergia odorifera attenuates inflammatory responses by inhibiting NF-κB activation via Nrf2-mediated heme oxygenase-1 expression. Phyther Res 28:1216–1223CrossRefGoogle Scholar
- Moon TC, Kim JC, Song SE, Suh SJ, Seo CS, Kim YK, Jin M, Yang JH, Son JK, Jahng Y, Kim CH, Chang HW (2008) Saucerneol D, a naturally occurring sesquilignan, inhibits LPS-induced iNOS expression in RAW264. 7 cells by blocking NF-κB and MAPK activation. Int Immunopharmacol 8:1395–1400CrossRefGoogle Scholar