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Archives of Pharmacal Research

, Volume 41, Issue 8, pp 830–837 | Cite as

Genome-wide evidences of bisphenol a toxicity using Schizosaccharomyces pombe

  • Dong-Myung Kim
  • Jeonghoon Heo
  • Dong Woo Lee
  • Mayumi Tsuji
  • Mihi Yang
Research Article

Abstract

To clarify reliable toxic mechanisms of bisphenol A (BPA), an endocrine disrupting chemical, we approached an alternative animal and whole genome analyses with the yeast knockout library (YKO) of Schizosaccharomyces pombe. As results, the 50% growth inhibition concentrations (GI50) of BPA was approximately 600 μM and the YKO—three step screening revealed the top 10 target candidate genes including dbp2, utp18, srs1, tif224, use1, qcr1, etc. The screening results were confirmed in human embryonic stem cell (hES)-derived hepatic cells and HepG2 human liver cancer cells. We found BPA down-regulated UQCRC, the human orthlog of S. pombe- qcr1, as a part of the mitochondrial respiratory chain, in HepG2 cells and hESs during cell differentiation into hepatic cells. Therefore, BPA may induce mitochondrial dysfunction and disruption of differentiation by suppressing UQCRC1.

Keywords

Bisphenol A Differentiation UQCRC1 Genome Mitochondria Yeast 

Notes

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) Grant funded by the Korea Government (MSIP) (No. 2011-0030074).

Compliance with ethical standards

Conflict of interest

No potential conflict of interest was reported by the authors.

Supplementary material

12272_2018_1058_MOESM1_ESM.pdf (71 kb)
Supplementary material 1 (PDF 71 kb)

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Copyright information

© The Pharmaceutical Society of Korea 2018

Authors and Affiliations

  • Dong-Myung Kim
    • 1
  • Jeonghoon Heo
    • 2
  • Dong Woo Lee
    • 3
  • Mayumi Tsuji
    • 4
  • Mihi Yang
    • 5
  1. 1.GPScreen TeamBioneer CorporationDaejeonRepublic of Korea
  2. 2.Department of Molecular Biology and Immunology, College of MedicineKosin UniversityBusanRepublic of Korea
  3. 3.Department of BioengineeringKonyang UniversityDaejeonRepublic of Korea
  4. 4.Department of Environmental Health, School of MedicineUniversity of Occupational and Environmental HealthKitakyushuJapan
  5. 5.Research Institute of Pharmaceutical Sciences (RIPS), Research Center for Cell Fate Control, College of PharmacySookmyung Women’s UniversitySeoulRepublic of Korea

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