Archives of Pharmacal Research

, Volume 41, Issue 2, pp 196–207 | Cite as

Dihydroxanthyletin-type coumarins from Angelica decursiva that inhibits the formation of advanced glycation end products and human recombinant aldose reductase

  • Md Yousof Ali
  • Hyun Ah JungEmail author
  • Susoma Jannat
  • Jae Sue ChoiEmail author
Research Article


The formation of advanced glycation end-products (AGE) and aldose reductase activity have been implicated in the development of diabetic complications. The present study was aimed to evaluate human recombinant aldose reductase (HRAR) and AGE inhibitory activity of seven natural dihydroxanthyletin-type coumarins, 4-hydroxy Pd-C-III (1), 4′-methoxy Pd-C-I (2), Pd-C-I (3), Pd-C-II (4), Pd-C-III (5), decursidin (6), and (+)-trans-decursidinol (7) from Angelica decursiva. Coumarins 1–7 showed potent HRAR and AGE inhibitory activities with ranges of IC50 values of 1.03–21.31 and 0.41–5.56 µM, respectively. In the kinetic study for HRAR enzyme inhibition, coumarins 1, 3, 4, and 7 were competitive-type inhibitors, 6 was a mixed-type inhibitor, whereas 2 and 5 were noncompetitive-type inhibitors. Furthermore, we also predicted the docking interactions of HRAR with coumarins 1–7 using AutoDock Vina, and as a result, the simulated enzyme-inhibitor complexes exhibited negative binding energies (Autodock Vina = − 9.6 to − 8.1 kcal/mol for HRAR), indicating a high affinity and tight binding capacity for the HRAR active site. Our results clearly indicate the potential HRAR and AGE formation inhibitory activities of dihydroxanthyletin-type coumarins, which could be further explored to develop therapeutic modalities for the treatment of diabetes and related complications.


A. decursiva Advanced glycation end-products Human recombinant aldose reductase Diabetic complication Molecular docking 



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) and funded by the Ministry of Science, ICT, and Future Planning (2014R1A1A3051684).


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Copyright information

© The Pharmaceutical Society of Korea 2017

Authors and Affiliations

  1. 1.Department of Food and Life SciencePukyong National UniversityBusanRepublic of Korea
  2. 2.Department of Food Science and Human NutritionChonbuk National UniversityJeonjuRepublic of Korea

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