Dysregulation of DNA-licensing is a hallmark for hereditary diseases, as well as for the development of tumour cells. Here we show how the combination of high-content imaging (HCl) with highly specific antibodies from Proteintech allows deeper insights into the timing of replication licensing following the loading of specific markers onto the DNA. Similar to flow cytometry, HCl can be used to investigate large quantities of cells whilst adding crucial structural information.
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Szczesna, K., Denninger, V. High-content imaging zur Identifikation von Replikationsfehlern. Biospektrum 26, 66–68 (2020). https://doi.org/10.1007/s12268-020-1322-5