Advertisement

BIOspektrum

, Volume 25, Issue 5, pp 510–512 | Cite as

Proteine mit ausreichend Häm-Kofaktor beladen

  • Kerstin Fiege
  • Nicole Frankenberg-DinkelEmail author
Wissenschaft · Methoden Rekombinante Proteine
  • 15 Downloads

Abstract

Heme proteins play an important role in several biological processes. For their functional analysis, recombinant proteins are widely used. However, the saturation of the proteins with the heme cofactor is often limited by the availability of heme inside the host cell. Several different methods to improve the recombinant production of heme proteins have been established, the most promising ones are being summarized here.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Literatur

  1. [1]
    Mills M, Payne SM (1995) Genetics and regulation of heme iron transport in Shigella dysenteriae and detection of an analogous system in Escherichia coli 0157:H7.1 Bacteriol 177:3004–3009CrossRefGoogle Scholar
  2. [2]
    Fiege K, Querebillo CI, Hildebrandt P et al. (2018) Improved method for the incorporation of heme cofactors into recombinant proteins using Escherichia coli Nissle 1917 Biochemistry 57:2747–2755PubMedGoogle Scholar
  3. [3]
    Woodard SI, Dailey HA (1995) Regulation of heme biosynthesis in Escherichia coli. Arch Biochem Biophys 316:110–115CrossRefPubMedGoogle Scholar
  4. [4]
    Wu C, Zhang I, Abu-Soud H et al. (1996) High-level expression of mouse inducible nitric oxide synthase in Escherichia coli requires coexpression with calmodulin. Biochem Biophys Res Commun 222:439–444CrossRefPubMedGoogle Scholar
  5. [5]
    Chen H, Jiang P (2019) Metabolic engineering of Escherichia coli for efficient biosynthesis of fluorescent phyco-biliprotein. Microb Cell Fact 18:58CrossRefPubMedPubMedCentralGoogle Scholar
  6. [6]
    Varnado CL, Goodwin DC (2004) System for the expression of recombinant hemoproteins in Escherichia coli. Protein Expr Purif 35:76–83CrossRefPubMedGoogle Scholar
  7. [7]
    Sonnenborn U, Schulze I (2009) The non-pathogenic Escherichia coli strain Nissle 1917 - features of a versatile probiotic. Microb Ecol Health Dis 21:122–158CrossRefGoogle Scholar
  8. [8]
    Grozdanov L, Raasch C, Schulze I et al. (2004) Analysis of the genome structure of the nonpathogenic probiotic Escherichia coli strain Nissle 1917. J Bacteriol:1 5432–5441CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 2019

Authors and Affiliations

  1. 1.Fachbereich BiologieAbteilung Mikrobiologie Technische Universität KaiserslauternKaiserslauternDeutschland

Personalised recommendations