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BIOspektrum

, Volume 24, Issue 6, pp 596–598 | Cite as

DNA-Extraktion aus mikrobiellen Umweltproben

  • Ramona Kuhn
  • Jörg Böllmann
  • Kathrin Krahl
  • Isaac Mbir Bryant
  • Marion Martienssen
Wissenschaft · Methoden Methodenvergleich
  • 6 Downloads

Abstract

We compared the efficiency of ten selected DNA extraction procedures for microbes published in recent literature using four different environmental samples. The suitability was assessed based on DNA yield, visual quality, as well as PCR applicability. Four out of ten extraction procedures were found applicable to all tested environmental samples. All tested procedures were cheaper compared to commercial DNA extraction kits.

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Copyright information

© Springer-Verlag GmbH Deutschland, ein Teil von Springer Nature 2018

Authors and Affiliations

  • Ramona Kuhn
    • 1
    • 2
  • Jörg Böllmann
    • 1
  • Kathrin Krahl
    • 1
  • Isaac Mbir Bryant
    • 1
  • Marion Martienssen
    • 1
  1. 1.Institut Für Umwelt- Und VerfahrenstechnikLehrstuhl Biotechnologie Der Wasseraufbereitung, Btu Cottbus-SenftenbergCottbusDeutschland
  2. 2.Institut für Umwelt- Und VerfahrenstechnikBrandenburgische Technische Universität Cottbus-SenftenbergCottbusDeutschland

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