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Acute In Vivo Functional Assessment of a Biodegradable Stentless Elastomeric Tricuspid Valve

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Degradable heart valves based on in situ tissue regeneration have been proposed as potentially durable and non-thrombogenic prosthetic alternatives. We evaluated the acute in vivo function, microstructure, mechanics, and thromboresistance of a stentless biodegradable tissue-engineered heart valve (TEHV) in the tricuspid position. Biomimetic stentless tricuspid valves were fabricated with poly(carbonate urethane)urea (PCUU) by double-component deposition (DCD) processing to mimic native valve mechanics and geometry. Five swine then underwent 24-h TEHV implantation in the tricuspid position. Echocardiography demonstrated good leaflet motion and no prolapse and trace to mild regurgitation in all but one animal. Histology revealed patches of proteinaceous deposits with no cellular uptake. SEM demonstrated retained scaffold microarchitecture with proteinaceous deposits but no platelet aggregation or thrombosis. Explanted PCUU leaflet thickness and mechanical anisotropy were comparable with native tricuspid leaflets. Bioinspired, elastomeric, stentless TEHVs fabricated by DCD were readily implantable and demonstrated good acute function in the tricuspid position.

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Double-component deposition


Epicardial echocardiography


Poly(carbonate urethane)urea


Scanning electron micrography


Tissue-engineered heart valve


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We would like to thank Joseph Hanke, Meegan Ambrose, and the rest of the excellent staff at the McGowan Center for Preclinical Studies for their assistance with animal care throughout this study. We would also like to thank the Center for Biological Imaging at the University of Pittsburgh for assistance with the scanning electron microscopy.


This work was supported by Wallace H. Coulter Foundation Translational Bioengineering Research Award, the Clinical and Translational Science Institute of the University of Pittsburgh, and the RiMED Foundation (grant 0057091).

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Correspondence to Antonio D’Amore.

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Conflict of Interest

William Wagner, Antonio D’Amore, and Vinay Badhwar filed utility patents pertaining to the technology described in this manuscript. All the other authors declare that they have no conflict of interest.

Human Subjects

No human studies were carried out by the authors for this article.

Animal Studies

All experiments described in this manuscript were approved by the Institutional Animal Care and Use Committee of the University of Pittsburgh (protocol number: 16047811). Animals were cared for in accordance with the Guide for the Care and Use of Laboratory Animals as published by the National Institute of Health.

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Read at the 99th Annual Meeting of the American Association for Thoracic Surgery on May 5, 2019.

Associate Editor Marat Fudim oversaw the review of this article

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Supplemental Figure 1

Operative implantation of the stentless tissue engineered tricuspid valve. (A) The valve is sutured in using a continuous polypropylene suture technique to the native tricuspid annulus on beating heart cardiopulmonary bypass. (B) The implanted tissue engineered tricuspid valve in the native tricuspid annulus (green arrow) (PNG 923 kb)

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Coyan, G.N., da Mota Silveira-Filho, L., Matsumura, Y. et al. Acute In Vivo Functional Assessment of a Biodegradable Stentless Elastomeric Tricuspid Valve. J. of Cardiovasc. Trans. Res. (2020).

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  • Tissue-engineered heart valve
  • Tricuspid valve replacement
  • Double-component deposition
  • Electrospinning
  • In vivo study
  • Biodegradable valve prosthesis