Preoperative miRNA-208a as a Predictor of Postoperative Complications in Children with Congenital Heart Disease Undergoing Heart Surgery

  • Keren Zloto
  • Tal Tirosh-Wagner
  • Yoav Bolkier
  • Omer Bar-Yosef
  • Amir Vardi
  • David Mishali
  • Gidi Paret
  • Yael Nevo-CaspiEmail author
Original Article


Major perioperative cardiovascular events are important causes of morbidity in pediatric patients with congenital heart disease who undergo reparative surgery. Current preoperative clinical risk assessment strategies have poor accuracy for identifying patients who will sustain adverse events following heart surgery. There is an ongoing need to integrate clinical variables with novel technology and biomarkers to accurately predict outcome following pediatric heart surgery. We tested whether preoperative levels of miRNAs-208a can serve as such a biomarker. Serum samples were obtained from pediatric patients immediately before heart surgery. MiRNA-208a was quantified by RQ-PCR. Correlations between the patient’s clinical variables and miRNA levels were tested. Lower levels of preoperative miRNA-208a correlated with and could predict the appearance of postoperative cardiac and inflammatory complications. MiRNA-208a may serve as a biomarker for the prediction of patients who are at risk to develop complications following surgery for the repair of congenital heart defects.


Biomarker miRNA Congenital heart disease Pediatric cardiac surgery Prediction of complications Preconditioning 



We thank Amisragas for their continuous support of the Department of Intensive Care.

Funding Information

This work was funded with institutional departmental funds.

Compliance with Ethical Standards

All procedures performed in this study involving human participants were in accordance with the ethical standards of the institutional research committee and with the 1964 Helsinki declaration and its later amendments. Ethical approval was obtained from the Ethics Committee of the Sheba Medical Center. No animal studies were carried out by the authors for this article.

Conflict of Interest

The authors declare that they have no conflict of interest.

Informed Consent

Informed consent was obtained from all individual participants or their parents/legal guardians included in the study.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pediatric Critical Care Medicine, Safra Children’s Hospital, Sheba Medical Center, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  2. 2.Department of Pediatric Cardiology, Safra Children’s Hospital, Sheba Medical Center, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  3. 3.Department of Pediatric Cardiac Intensive Care, Safra Children’s Hospital, Sheba Medical Center, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael
  4. 4.Department of Pediatric Cardiac Surgery, Safra Children’s Hospital, Sheba Medical Center, Sackler Faculty of MedicineTel-Aviv UniversityTel-AvivIsrael

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