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lncRNA HOTAIR Protects Myocardial Infarction Rat by Sponging miR-519d-3p

  • Dongying Zhang
  • Bingjian Wang
  • Min Ma
  • Kun Yu
  • Qing Zhang
  • Xiwen ZhangEmail author
Original Article

Abstract

Myocardial infarction (MI) is a cardiovascular disease with high morbidity and mortality. In this study, we focused on exploring the roles and underlying regulatory mechanisms of Hox transcript antisense intergenic RNA (HOTAIR) and miR-519d-3p in myocardial infarction. To comprehensively understand the role of microRNA in MI rat, we construct MI rat model by permanent ligation of the left anterior descending (LAD) coronary artery. Cardiac troponin I and creatine kinase-MB concentration measured by ELISA and infract size of heart section analyzed by TTC staining were served as evaluation indicators to confirmed the established model. Based on the bioinformatics assay and qRT-PCR, we found that the expression of miR-519d-3p was upregulated remarkably. Dual-luciferase reporter assays were performed to investigate the interaction of lncRNA HOTAIR and miR-519d-3p. In order to investigate the potential mechanism of lncRNA HOTAIR and miR-519d-3p, flow cytometry was applied to measure apoptotic cardiomyocytes and western blot was used to detect expressions of apoptotic related protein Bax, Bcl-2, and caspase-3 in cardiomyocytes in vitro and myocardial infraction in vivo. Downregulating miR-519d-3p or overexpressing HOTAIR alleviated MI or hypoxia-induced cardiomyocytes apoptosis. Taken together, our results showed that the interaction of miR-519d-3p and HOTAIR can protect MI and hypoxia-induced cardiomyocytes apoptosis, providing the potential therapeutic target for MI treatment.

Keywords

Myocardial infarction miR-519d-3p HOTAIR Cardiomyocytes apoptosis Coronary heart disease 

Abbreviations

CHD

Coronary heart disease

CK-MB

Creatine kinase-MB

cTn

Cardiac troponin

ECG

Electrocardiographic

HF

Heart failure

LAD

Left anterior descending

LV

Left ventricular

MI

Myocardial infarction

NSTEMI

Non-ST -segment elevation myocardial infarction

PRC2

Polycomb repressive complex 2

STEMI

ST -segment elevation myocardial infarction

TTC

Triphenyltetrazolium chloride

Notes

Source of Funding

This work was supported by Jiangsu Province Health and Family Planning Commission Scientific Research Project (H2017011).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no competing interests.

Ethical Approval

All institutional and national guidelines for the care and use of laboratory animals were followed and approved by the Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical University.

Informed Consent Statement

No human studies were carried out by the authors for this article.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Dongying Zhang
    • 1
  • Bingjian Wang
    • 1
  • Min Ma
    • 2
  • Kun Yu
    • 1
  • Qing Zhang
    • 1
  • Xiwen Zhang
    • 1
    Email author
  1. 1.Department of CardiologyThe Affiliated Huaian No. 1 People’s Hospital of Nanjing Medical UniversityHuaianChina
  2. 2.Department of CardiologyThe Sixth People’s Hospital of ChengduChengduChina

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