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Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2

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Abstract

Exosomes are small-sized vesicles that can be released from cells into the serum. Exosomes play important roles in regulating many biological processes including cell proliferation, apoptosis, cell cycle, and metabolism. However, the roles and mechanisms of plasma exosomes in the apoptosis of rat H9C2 cardiomyocytes are largely unknown. In this study, we isolated plasma exosomes as confirmed by the marker protein CD63. Using flow cytometry and western blot analysis, we found that exosomes attenuated hydrogen peroxide (H2O2)-induced apoptosis and improved survival of rat H9C2 cardiomyocytes. Furthermore, the anti-apoptosis effects of serum exosomes in rat H9C2 cardiomyocytes were mediated by the activation of ERK1/2 signaling pathway. These data indicated that plasma exosomes had the protective effects against cardiomyocyte apoptosis and might be a novel therapy strategy for myocardial injury.

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Funding

This work was supported by the grants from the CAMS Innovation Fund for Medical Sciences (CIFMS, 2016-12M-1-006), National Natural Science Foundation of China (81700228 to Y Xie, 81570332 to L Zhou) and the grant from Jiangsu Province’s Key Provincial Talents Program (ZDRCA2016019).

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  1. Pengfei Li, Zhuyuan Liu and Yuan Xie contributed equally to this work.

Authors and Affiliations

  1. Department of Cardiology, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China

    Pengfei Li, Zhuyuan Liu, Huanyu Gu, Qiying Dai & Lei Zhou

  2. Department of Cardiology, Shanghai Tongji Hospital, Tongji University School of Medicine, Shanghai, 200065, China

    Yuan Xie

  3. Department of Cardiology, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, 301 Middle Yanchang Road, Shanghai, 200072, China

    Jianhua Yao

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  1. Pengfei Li
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Correspondence to Jianhua Yao or Lei Zhou.

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All human investigations conformed to the principles outlined in the Declaration of Helsinki and were approved by the institutional review committees of Nanjing Medical School. All participants provided written informed consent when they were enrolled in this study.

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Associate Editor Junjie Xiao oversaw the review of this article

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Li, P., Liu, Z., Xie, Y. et al. Serum Exosomes Attenuate H2O2-Induced Apoptosis in Rat H9C2 Cardiomyocytes via ERK1/2. J. of Cardiovasc. Trans. Res. 12, 37–44 (2019). https://doi.org/10.1007/s12265-018-9791-3

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