Neuroscience Bulletin

, Volume 35, Issue 6, pp 1035–1044 | Cite as

Virus-Mediated Overexpression of ETS-1 in the Ventral Hippocampus Counteracts Depression-Like Behaviors in Rats

  • Hanjiang Luo
  • Zijin Liu
  • Bo Liu
  • Hui Li
  • Yutao YangEmail author
  • Zhi-Qing David XuEmail author
Original Article


ETS-1 is a transcription factor that is a member of the E26 transformation-specific (ETS) family. Galanin receptor 2 (GalR2), a subtype of receptors of the neuropeptide galanin, has been shown to have an antidepressant-like effect after activation in rodents. Our previous study has shown that overexpression of ETS-1 increases the expression of GalR2 in PC12 phaeochromocytoma cells. However, whether ETS-1 has an antidepressant-like effect is still unclear. In this study, we found that chronic mild stress (CMS) decreased the expression of both ETS-1 and GalR2 in the ventral hippocampus of rats. Meanwhile, we demonstrated that overexpression of ETS-1 increased the expression of GalR2 in primary hippocampal neurons. Importantly, we showed that overexpression of ETS-1 in the ventral hippocampus counteracted the depression-like behaviors of CMS rats. Furthermore, we found that overexpression of ETS-1 increased the level of downstream phosphorylated extracellular signal-regulated protein kinases 1 and 2 (p-ERK1/2) of GalR2 in the ventral hippocampus of CMS rats. Taken together, our findings suggest that ETS-1 has an antidepressant-like effect in rats, which might be mediated by increasing the level of GalR2 and its downstream p-ERK1/2 in the ventral hippocampus.


GalR2 ETS-1 Depression Chronic mild stress 



This work was supported by grants from the National Natural Science Foundation of China (31271154, 31171032, and 81671345), the Beijing Natural Science Foundation (7162016), the Special Project on Natural Chronic Non-infectious Diseases (2016YFC1307202), and the Beijing Municipal Science and Technology Commission (Z181100001518001).

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

12264_2019_412_MOESM1_ESM.pdf (108 kb)
Supplementary material 1 (PDF 107 kb)


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Copyright information

© Shanghai Institutes for Biological Sciences, CAS 2019

Authors and Affiliations

  1. 1.Department of Neurobiology, Beijing Key Laboratory of Neural Regeneration and Repair, Beijing Laboratory of Major Brain Disorders (Ministry of Science and Technology), Beijing Institute for Brain DisordersCapital Medical UniversityBeijingChina
  2. 2.Department of AnatomyCapital Medical UniversityBeijingChina

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