Advertisement

Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila

Abstract

The negative-geotaxis climbing assay is used to efficiently study aging and neurodegeneration in Drosophila. To make it suitable for large-scale study, a method called the rapid iterative negative geotaxis (RING) assay has been established by simultaneously photographing the climbing of multiple groups of flies when they are manually tapped down in test tubes. Here, we automated the assay by using a well-controlled electric motor to drive the tapping, and a homemade program to analyze the climbing height of flies. Using the automated RING (aRING) assay, we found that the climbing ability of a strain of wild-type flies, males in particular, declined rapidly before day 21 after eclosion, but slowly from day 21 to 35. We also found that the expression of arctic mutant Aβ42 accelerated the age-dependent decline in the climbing ability of flies. Moreover, using aRING, we examined the effect of third chromosome deficiencies on the accelerated locomotor decline in Aβ42-expressing flies, and isolated 7 suppressors and 15 enhancers.

This is a preview of subscription content, log in to check access.

Access options

Buy single article

Instant unlimited access to the full article PDF.

US$ 39.95

Price includes VAT for USA

References

  1. [1]

    Bilen J, Bonini NM. Drosophila as a model for human neurodegenerative disease. Annu Rev Genet 2005, 39: 153–171.

  2. [2]

    Marsh JL, Thompson LM. Drosophila in the study of neurodegenerative disease. Neuron 2006, 52: 169–178.

  3. [3]

    Pandey UB, Nichols CD. Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery. Pharmacol Rev 2011, 63: 411–436.

  4. [4]

    Jones MA, Grotewiel M. Drosophila as a model for age-related impairment in locomotor and other behaviors. Exp Gerontol 2011, 46: 320–325.

  5. [5]

    Feany MB, Bender WW. A Drosophila model of Parkinson’s disease. Nature 2000, 404: 394–398.

  6. [6]

    Gargano JW, Martin I, Bhandari P, Grotewiel MS. Rapid iterative negative geotaxis (RING): a new method for assessing age-related locomotor decline in Drosophila. Exp Gerontol 2005, 40: 386–395.

  7. [7]

    Podratz JL, Staff NP, Boesche JB, Giorno NJ, Hainy ME, Herring SA, et al. An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster. Fly (Austin) 2013, 7: 187–192.

  8. [8]

    Zhao XL, Wang WA, Tan JX, Huang JK, Zhang X, Zhang BZ, et al. Expression of beta-amyloid induced age-dependent presynaptic and axonal changes in Drosophila. J Neurosci 2010, 30: 1512–1522.

  9. [9]

    Rhodenizer D, Martin I, Bhandari P, Pletcher SD, Grotewiel M. Genetic and environmental factors impact age-related impairment of negative geotaxis in Drosophila by altering age-dependent climbing speed. Exp Gerontol 2008, 43: 739–748.

  10. [10]

    Lin JY, Wang WA, Zhang X, Liu HY, Zhao XL, Huang FD. Intraneuronal accumulation of Abeta42 induces age-dependent slowing of neuronal transmission in Drosophila. Neurosci Bull 2014, 30: 185–190.

  11. [11]

    Huang JK, Ma PL, Ji SY, Zhao XL, Tan JX, Sun XJ, et al. Age-dependent alterations in the presynaptic active zone in a Drosophila model of Alzheimer’s Disease. Neurobiol Dis 2013, 51: 161–167.

  12. [12]

    Mossmann D, Vogtle FN, Taskin AA, Teixeira PF, Ring J, Burkhart JM, et al. Amyloid-beta peptide induces mitochondrial dysfunction by inhibition of preprotein maturation. Cell Metab 2014, 20: 662–669.

Download references

Author information

Correspondence to Wen-An Wang or Fu-De Huang.

Electronic supplementary material

Supplementary material, approximately 10.2 KB.

Supplementary material, approximately 3.98 MB.

Supplementary material, approximately 3.98 MB.

Supplementary material, approximately 4.23 MB.

Supplementary material, approximately 4.23 MB.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Liu, H., Han, M., Li, Q. et al. Automated rapid iterative negative geotaxis assay and its use in a genetic screen for modifiers of Aβ42-induced locomotor decline in Drosophila . Neurosci. Bull. 31, 541–549 (2015) doi:10.1007/s12264-014-1526-0

Download citation

Keywords

  • RING
  • negative geotaxis
  • giant fiber
  • age-dependent
  • locomotor decline
  • Drosophila
  • beta amyloid