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Biotechnology and Bioprocess Engineering

, Volume 24, Issue 3, pp 552–559 | Cite as

Ectopic Overexpression of Teff Grass (Eragrostis tef) Phi-class Glutathione S-transferase 1 (EtGSTF1) Enhances Prokaryotic Cell Survivability against Diverse Abiotic Stresses

  • Ki-Won Lee
  • Serim Hong
  • Md. Atikur Rahman
  • Hee Chung Ji
  • Joon-Yung Cha
  • Chris Stephen Jones
  • Daeyoung SonEmail author
  • Sang-Hoon LeeEmail author
Research Paper

Abstract

The glutathione S-transferases (GSTs) are encoded by a large gene family and well conserved in all living organisms; however they have evolved and are classified clearly according to each kingdom. GSTs are a dimeric protein that has been reported to maintain redox homeostasis in cells, and to protect organisms against oxidative damage. Recently, we isolated a GST coding gene from stress-treated teff grass (Eragrostis tef) and identified it as a plant-specific phi class GST (EtGSTF1) possessing conserved phi class-specific GST N- and C-terminal domains, GSH binding site, substrate binding pocket, and dimer interface. We found that overexpression of plant-specific phi class EtGSTF1 confers diverse abiotic stress tolerances including salt, osmotic, and heat stresses in E. coli which does not possess phi class GSTs. In addition, EtGSTF1 expression helps the E. coli cells tolerate arsenic (As)-induced cell toxicity. Collectively, although plantae and prokaryotae have differentiated a few billions of years ago, the plant-specific phi class EtGSTF1 could protect prokaryotic organisms by detoxification of molecules under diverse abiotic stresses.

Keywords

abiotic stress arsenic ectopic overexpression glutathione S-transferases teff grass 

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Notes

Acknowledgments

This research work was supported by the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ012187)”. This study was also supported by Postdoctoral Fellowship Program of National Institute of Animal Science funded by Rural Development Administration (RDA), Republic of Korea.

Supplementary material

12257_2018_495_MOESM1_ESM.pdf (55 kb)
Supplementary Fig. 1. Sequence of teff grass GSTF1 (EtGSTF1). Nucleotide and deduced amino acid sequence of EtGSTF1. Numbers at left indicate the nucleotide (up) and amino acid residue (down) position.

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer 2019

Authors and Affiliations

  • Ki-Won Lee
    • 1
  • Serim Hong
    • 2
  • Md. Atikur Rahman
    • 1
  • Hee Chung Ji
    • 1
  • Joon-Yung Cha
    • 3
  • Chris Stephen Jones
    • 4
  • Daeyoung Son
    • 2
    • 3
    Email author
  • Sang-Hoon Lee
    • 5
    Email author
  1. 1.Grassland & Forages DivisionNational Institute of Animal Science, Rural Development AdministrationCheonanKorea
  2. 2.Department of Plant MedicineGyeongsang National UniversityJinjuKorea
  3. 3.Division of Applied Life Science (BK21 Plus), Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinjuKorea
  4. 4.Feed and Forage BiosciencesInternational Livestock Research InstituteAddis AbabaEthiopia
  5. 5.Animal Genetic Resources Research CenterNational Institute of Animal Science, Rural Development AdministrationNamwonKorea

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