Resistance and Proteomic Response of Microalgae to Ionizing Irradiation
- 7 Downloads
Microalgae have been drawing much attention as a platform for food supplements and biofuel production. Advance molecular tools are not available for manipulating microalgae, and therefore, methods for strain improvement mostly depend on random mutation. Radiation is frequently used mutagen in plant as well as microalgae breeding methods. In this study, the resistance of 7 microalgae species to ionizing irradiation was measured. To monitor the growth of microalgae, optical density and staining methods were used. Based on the D10 values, the dose required to reduce one log cycle of the cell population, Chlorella protothecoides, Zygnema circumcarinatum, and Spirogyra varians were shown to be highly resistant to ionizing radiation. The changes in protein expression levels in S. varians were further investigated. Using 2-dimensional electrophoresis and protein identification, it was shown that some proteins involved in energy and glyceride metabolisms were up-regulated. These results provide fundamental insights into metabolic changes that occur in a microalga species upon exposure to ionizing irradiation.
Keywordsionizing irradiation microalgae proteome resistance
Unable to display preview. Download preview PDF.
- 2.Hong, S. J., Y. S. Park, M. A. Han, Z. H, Kim, B. K. Cho, H. Lee, H. K. Choi, and C. G. Lee (2017) Enhanced production of fatty acids in three strains of microalgae using a combination of nitrogen starvation and chemical inhibitors of carbohydrate synthesis. Biotechnol. Bioproc. Eng. 22: 60–67.CrossRefGoogle Scholar
- 6.Garcia, M. M., B. W. Brooks, R. B. Stewart, W. Dion, J. R. Trudel, and T. Ouwerkerk (1987) Evaluation of gamma radiation levels for reducing pathogenic bacteria and fungi in animal sewage and laboratory effluents. Can. J. Vet. Res. 51: 285–289.Google Scholar
- 17.Herburger, H. and A. Holzinger (2015) Localization and quantification of callose in the streptophyte green algae Zygnema and Klebsormidium: Correlation with desiccation tolerance. Plant and Cell Physiology 56: 2259–2270.Google Scholar
- 26.Wei, S. J., C. S. Trempus, R. C. Ali, L. A. Hansen, and R. W. Tennant (2004) 12–O–tetradecanoylphorbol–13–acetate and UV radiationinduced nucleoside diphosphate protein kinase B mediates neoplastic transformation of epidermal cells. J. Biol. Chem. 279: 5993–6004.Google Scholar