Biotechnology and Bioprocess Engineering

, Volume 24, Issue 2, pp 366–374 | Cite as

Enzymatic and Microbial Biosynthesis of Novel Violacein Glycosides with Enhanced Water Solubility and Improved Anti-nematode Activity

  • Yu Jeong Lee
  • Puspalata Bashyal
  • Ramesh Prasad PandeyEmail author
  • Jae Kyung SohngEmail author
Research Paper


Violacein, a microbial metabolite with multiple applications, was produced in Escherichia coli, and glycodiversified using purified Bacillus glycosyltransferase (YjiC) enzyme with glucose, galactose, and N-acetylglucosamine, to generate five novel O-glycoside derivatives. One of the glucose-conjugated derivatives, violacein 5′-O-glucoside, was produced from engineered E. coli harboring entire violacein biosynthetic gene cluster (VioABCDE) and a glycosyltransferase gene (yjiC) in tryptophan supplemented TB medium. Violacein 5′-O-glucoside gained anti-nematodal activity against pine wood nematode Bursaphelenchus xylophilus, a causative agent of pine wilt disease. Moreover, the conjugation of sugar moieties in violacein enhanced water solubility. Violacein 5′-O-diglucoside was completely retained in water fraction, while its aglycone parent molecule was completely insoluble in water.


violacein glycodiversification anti-nematode novel glycosides 


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This work was supported by a grant from the Next-Generation BioGreen 21 Program (SSAC, grant#: PJ013137), Rural Development Administration, Republic of Korea.

Supplementary material

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Copyright information

© The Korean Society for Biotechnology and Bioengineering and Springer 2019

Authors and Affiliations

  1. 1.Department of Life Science and Biochemical EngineeringSun Moon UniversityAsanKorea
  2. 2.Department of Pharmaceutical Engineering and BiotechnologySun Moon UniversityAsanKorea

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